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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [ChIPbisSeq]

ABSTRACT: We report the application of ChIP bisulfite sequencing (ChIPbisSeq) to establish the methylation state of DNA bound to RNApol2 phosphorylated in Ser5 (RNAPol2Ser5) in the mouse cortex. We first profiled the RNAPol2Ser5 binding using regular ChIPSeq from 45 million raw read pairs (31 million unique pairs were aligned to the genome). Then we used bisulfite converted DNA immunoprecipitated with an antibody against RNAPol2Ser5 (87 million raw read pairs, 38 million unique read pairs aligned to the genome) to map RNAPol2Ser5 sites and to establish the methylation state at these sites.

ORGANISM(S): Mus musculus

PROVIDER: GSE60657 | GEO | 2015/09/01

SECONDARY ACCESSION(S): PRJNA259205

REPOSITORIES: GEO

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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [ChIPbisSeq]

Project description:We report the application of ChIP bisulfite sequencing (ChIPbisSeq) to establish the methylation state of DNA bound to RNApol2 phosphorylated in Ser5 (RNAPol2Ser5) in the mouse cortex. We first profiled the RNAPol2Ser5 binding using regular ChIPSeq from 45 million raw read pairs (31 million unique pairs were aligned to the genome). Then we used bisulfite converted DNA immunoprecipitated with an antibody against RNAPol2Ser5 (87 million raw read pairs, 38 million unique read pairs aligned to the genome) to map RNAPol2Ser5 sites and to establish the methylation state at these sites. Examination of methylation state at RNAPol2Ser5 binding sites in the mouse cortex.

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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-09-01 | GSE60658 | GEO

DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [MeDIP]

Project description:We measured the methylation levels of gene promoters of cerebral cortex of mice. We used MeDIP-array, immunoprecipitating genomic DNA with an antibody against methylated DNA and measuring methylation levels using custom arrays. The input DNA was used as control.

2015-09-01 | GSE60656 | GEO

Large conserved domains of low DNA methylation maintained by 5-hydroxymethycytosine and Dnmt3a [Bisulfite-Seq]

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