Gene expression alteration in chondrocyte by HES1 overexpression
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE60737 | GEO | 2015/03/02
SECONDARY ACCESSION(S): PRJNA259379
REPOSITORIES: GEO
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Project description:To investigate target molecules of HES1, we transduced GFP or HES1 into mouse chondrocyte cell line ATDC5, and performed the microarray analysis. HES1 overexpression increased inflammation-related genes including Il6 and Il1rl1. We established stable ATDC5 cells overexpressing GFP or HES1 by lentiviral transduction. We harvested mRNA from these cells, and performed microarray analysis.
2015-03-02 | E-GEOD-60737 | biostudies-arrayexpress
Project description:To investigate loss-of-function of the C/EBP family members, we used A-CEBP which exerts a dominant-negative effect against all CEBPs. DOX-inducible overexpression of A-CEBP into mouse chondrocyte cell line ATDC5 increased expressions of early differentiation markers, decreased those of late differentiation markers. In addition, A-CEBP altered many genes related with skeletal development, cartilage, cell cycle, inflammation and apoptosis. We established stable ATDC5 cells which express GFP or A-CEBP by DOX induction. We started differentiation of these cells by ITS supplement immediately after DOX induction, harvested mRNA after 3 weeks, and performed microarray analysis.
2014-11-01 | E-GEOD-60651 | biostudies-arrayexpress
Project description:To investigate loss-of-function of the C/EBP family members, we used A-CEBP which exerts a dominant-negative effect against all CEBPs. DOX-inducible overexpression of A-CEBP into mouse chondrocyte cell line ATDC5 increased expressions of early differentiation markers, decreased those of late differentiation markers. In addition, A-CEBP altered many genes related with skeletal development, cartilage, cell cycle, inflammation and apoptosis.
2014-11-01 | GSE60651 | GEO
Project description:BACKGROUND: We previously demonstrated that temperature-related microwave ablation (MWA) can safely regulate vertebral growth plates of piglets. Therefore, the present study was designed to investigate the effects of different temperatures on chondrocyte proliferation and the molecular mechanisms involved in vitro. METHODS: After treatment for 10 min using different temperatures (37°C, 40°C, 42°C, 44°C, 46°C, 48°C, or 50°C), CCK-8 assay examined the activity of ATDC5 cells at 12 hr. RNA-seq identified differentially expressed genes (DEGs) and the hub genes in ATDC5 cells treated at 37°C, 40°C and 44°C. RT-qPCR verified the differential expression of hub genes in ATDC5 cells. RESULTS: Compared with 37°C, stimulation at 40°C significantly enhanced the activity of ATDC5 cells, and 40°C had no significant effect. In addition, stimulation at 44°C, 46°C, 48°C, or 50°C exhibited the opposite pattern, and ATDC5 cells were particularly less than 50% active after treatment at 46°C, 48°C, or 50°C. Differential expression analysis revealed the presence of 179, 374 and 221 DEGs in 40°C vs. 37°C, 44°C vs. 37°C, and 44°C vs. 40°C, respectively. Enrichment analysis indicated that these DEGs predominantly regulate proliferation, differentiation, necrosis, inflammatory and immune responses, and ECM synthesis/degradation, and that they are associated with the Ras, PI3K/AKT, mTOR, cAMP, and MAPK pathways. Furthermore, Agt, Hspa1a, Hspb1, and Nlrc4 were hub genes in DEGs, and RT-qPCR confirmed that the mRNA expression patterns of these hub genes in ATDC5 cells were essentially consistent with the RNA-seq results. CONCLUSION: Temperature-mediated chondrocyte proliferation was associated with Ras, PI3K/AKT, mTOR, cAMP, and MAPK pathways, and Agt, Hspa1a, Hspb1, and Nlrc4 may be the key regulatory genes.
2024-12-10 | GSE269585 | GEO
Project description:Chondrocytes can potentially perceive mechanical stimuli via Piezo channels. We investigated the effect of the Piezo1 agonist Yoda1 on chondrocyte-like ATDC5 cells. Chondrocytes can potentially perceive mechanical stimuli via Piezo channels. We investigated the effect of the Piezo1 agonist Yoda1 on chondrocyte-like ATDC5 cells. Chondrocytes can potentially perceive mechanical stimuli via Piezo channels. We investigated the effect of the Piezo1 agonist Yoda1 on chondrocyte-like ATDC5 cells. Chondrocytes can potentially perceive mechanical stimuli via Piezo channels. We investigated the effect of the Piezo1 agonist Yoda1 on chondrocyte-like ATDC5 cells. We used microarray analysis to detail the global gene expression of ATDC5 cells in response to 6 hours of treatment with 5µM Yoda1.
2024-02-28 | GSE230071 | GEO
Project description:High levels of Hes1 expression are frequently found in BCR-ABL-positive chronic myelogenous leukemia in blast crisis (CML-BC). In mouse bone marrow transplantation (BMT) models, co-expression of BCR-ABL and Hes1 induces CML-BC–like disease; however the underlying mechanism remained elusive. Here, based on gene expression analysis, we show that MMP-9 is upregulated by Hes1 in common myeloid progenitors (CMPs). Analysis of promoter activity demonstrated that Hes1 upregulated MMP-9 by activating NF-kB. Analysis of 20 samples from CML-BC patients showed that MMP-9 was highly expressed in three, with two exhibiting high levels of Hes1 expression. Interestingly, MMP-9 deficiency impaired the cobblestone area-forming ability of CMPs expressing BCR-ABL and Hes1 that were in conjunction with a stromal cell layer. In addition, these CMPs secreted MMP-9, promoting the release of soluble Kit-ligand (sKitL) from stromal cells, thereby enhancing proliferation of the leukemic cells. In accordance, mice transplanted with CMPs expressing BCR-ABL and Hes1 exhibited high levels of sKitL as well as MMP-9 in the serum. Importantly, MMP-9 deficiency impaired the development of CML-BC–like disease induced by BCR-ABL and Hes1 in mouse BMT models. The present results suggest that Hes1 promotes the development of CML-BC, partly through MMP-9 upregulation in leukemic cells. Common myeloid progenitors (CMPs; Lineage negative, c-Kit positive, Sca-1 negative, Fc-gamma-receptor low, CD34 positive fraction) were sorted with a FACSAria cell sorter (Becton Dickinson). Retroviruses were generated by transfecting Plat-E packaging cells with retrovirus vector pMYs-Hes1-IRES-GFP or empty vector (pMYs-IRES-GFP) using FuGENE 6 (Roche Diagnostics). Infection of retrovirus harboring Hes1 (pMYs-Hes1-IRES-GFP) or empty vector (pMYs-IRES-GFP) into progenitors was performed using RetroNectin (Takara Bio). Hes1-transfected CMPs and Mock-transduced CMPs were isolated 36 hours after infection with a FACSAria cell sorter. One sample of Hes1-transfected CMPs and one sample of mock-transduced CMPs were analyzed with GeneChip Mouse Genome 430 2.0 Array.
2014-05-01 | E-GEOD-56921 | biostudies-arrayexpress
Project description:To investigate the protective effect of Fascin1 inhibitor NP-G2-044 against chondrocyte dedifferentiation, we stimulated murine chondrogenic cell line ATDC5 cells with IL-1β to induce dedifferentiation, and with or without NP-G2-044 treatment. Gene expression profiling analysis was performed using data obtained from RNA-seq of three independent experiments.
2023-05-27 | GSE232611 | GEO
Project description:We performed genome-wide transcriptome profiling in stable Kmt2d-/- (bi-allelic deletion of the catalytic SET domain) and Kmt2d+/+ ATDC5 chondrocyte cell lines 7 days after induction of differentiation and in stable Kmt2d-/- and Kmt2d+/+ undifferentiated ATDC5 cells.
2019-10-09 | GSE129365 | GEO
Project description:To investigate the role of HES1 during glucocorticoid signaling in the liver, we employed whole-genome microarray expressions in liver mRNA extracted from control and Hes1-liver knockout male mice (HESKOL) that had been adrenalectomized to remove endogenous glucocorticoids.
2014-01-08 | GSE52106 | GEO
Project description:In the current investigation, an RNA-seq analysis was conducted to examine the differentially expressed genes in old ADSCs (O-ADSCs) following the overexpression of HES1. The findings indicated that 448 genes exhibited increased levels, while 865 genes showed decreased levels in the oe-HES1 O-ADSCs compared to the oe-NC O-ADSCs. The GO analysis revealed that these genes are primarily localized in the nucleus and cytoplasm, and are involved in activities such as protein binding and DNA binding. Furthermore, they are associated with processes related to cell adhesion and the regulation of transcription by RNA polymerase II. The KEGG analysis indicated enrichment in pathways such as the NOD-like receptor signaling pathway, TNF signaling pathway, IL-17 signaling pathway, and PI3K-Akt signaling pathway.
2024-11-13 | GSE270037 | GEO