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Expression data from follicle associated epithelium (FAE) of small intestine in wild type C57BL/6 mice or mice depleted with RBP-J (by VillinCre-Rbpjflox/flox)

ABSTRACT: To investigate the role of RBP-J in regulating epithelial cell differention in small intestine, we isolated FAE from mice depleted with RBP-J

ORGANISM(S): Mus musculus

PROVIDER: GSE61058 | GEO | 2014/09/04

SECONDARY ACCESSION(S): PRJNA260169

REPOSITORIES: GEO

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Project description:BackgroundThere is increasing appreciation for sexually dimorphic effects, but the molecular mechanisms underlying these effects are only partially understood. In the present study, we explored transcriptomics and epigenetic differences in the small intestine and colon of prepubescent male and female mice. In addition, the microbiota composition of the colonic luminal content has been examined.MethodsAt postnatal day 14, male and female C57BL/6 mice were sacrificed and the small intestine, colon and content of luminal colon were isolated. Gene expression of both segments of the intestine was analysed by microarray analysis. DNA methylation of the promoter regions of selected sexually dimorphic genes was examined by pyrosequencing. Composition of the microbiota was explored by deep sequencing.ResultsSexually dimorphic genes were observed in both segments of the intestine of 2-week-old mouse pups, with a stronger effect in the small intestine. Amongst the total of 349 genes displaying a sexually dimorphic effect in the small intestine and/or colon, several candidates exhibited a previously established function in the intestine (i.e. Nts, Nucb2, Alox5ap and Retnl?). In addition, differential expression of genes linked to intestinal bowel disease (i.e. Ccr3, Ccl11 and Tnfr) and colorectal cancer development (i.e. Wt1 and Mmp25) was observed between males and females. Amongst the genes displaying significant sexually dimorphic expression, nine genes were histone-modifying enzymes, suggesting that epigenetic mechanisms might be a potential underlying regulatory mechanism. However, our results reveal no significant changes in DNA methylation of analysed CpGs within the selected differentially expressed genes. With respect to the bacterial community composition in the colon, a dominant effect of litter origin was found but no significant sex effect was detected. However, a sex effect on the dominance of specific taxa was observed.ConclusionsThis study reveals molecular dissimilarities between males and females in the small intestine and colon of prepubescent mice, which might underlie differences in physiological functioning and in disease predisposition in the two sexes.

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Expression data from follicle associated epithelium (FAE) of small intestine in wild type C57BL/6 mice or mice depleted with RBP-J (by VillinCre-Rbpjflox/flox)

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