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Dilution and amplification control study

ABSTRACT: Mouse adult female brain’s cortex (C57BL/6, Charles River Laboratories, Inc.) was isolated and stored immediately at -80°C. Subsequently, the mRNA (15µg) was isolated using TRIzol Reagent and MicroFastTrack 2.0 Kit (Invitrogen). A Sample of 5µg was assessed on Affymetrix Mouse 430.2 array. Aliquots from the leftovers of the same cortical mRNA were diluted to single-cell RNA levels (0.1, 1, and 10 pg) and independently aRNA amplified for a total of 2 and 4 rounds and assessed on Affymetrix Mouse 430.2 arrays.

ORGANISM(S): Mus musculus

PROVIDER: GSE61081 | GEO | 2014/10/14

SECONDARY ACCESSION(S): PRJNA260207

REPOSITORIES: GEO

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Dilution and amplification control study

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