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Microarray-based gene expression of rabbit basilar artery smooth muscle cells exposed to hemodynamic insult

ABSTRACT: High-flow causes the remodeling of arteries, in which smooth muscle cells play an important role. To know the profile of smooth muscle gene expression under high-flow conditions in vivo, flow of rabbit basilar artery was increased by ligation of both common carotid arteries. Microarrays were performed to profile the gene expression of smooth muscle cells isolated from rabbit basilar artery. Expression profiles indicate 43603 differentially expressed genes in smooth muscle cells exposed to high-flow insult compared with the sham control, of which 1470 genes were upregulated and 780 genes downregulated using 2 fold-changes and P<0.05 as a cut-off.

ORGANISM(S): Oryctolagus cuniculus

PROVIDER: GSE61212 | GEO | 2016/06/30

SECONDARY ACCESSION(S): PRJNA260513

REPOSITORIES: GEO

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Microarray-based gene expression of rabbit basilar artery smooth muscle cells exposed to hemodynamic insult

Project description:High-flow causes the remodeling of arteries, in which smooth muscle cells play an important role. To know the profile of smooth muscle gene expression under high-flow conditions in vivo, flow of rabbit basilar artery was increased by ligation of both common carotid arteries. Microarrays were performed to profile the gene expression of smooth muscle cells isolated from rabbit basilar artery. Expression profiles indicate 43603 differentially expressed genes in smooth muscle cells exposed to high-flow insult compared with the sham control, of which 1470 genes were upregulated and 780 genes downregulated using 2 fold-changes and P<0.05 as a cut-off. Bilateral common carotid arteries of female New Zealand White rabbits were ligated to increase vascular flow.The control group was performed the same procedure to expose the CCAs without ligation. Rabbits were euthanized at day 5 after ligation or exposure of bilateral CCAs in both groups (n=3 for each group). The rabbits used and all procedures in this study were approved by the local Institutional Animal Care and Use Committee. Smooth muscle cells were isolated. After euthanization of rabbits, the whole basilar arteries were removed. The arteries were cleaned in PBS buffer,cannulated and perfused at a constant flow with a cocktail which contains PBS and 0.4 mg/ml elastase (Sigma) and 1 mg/ml collagenase (type 1A, Sigma). After an incubation time of 45 min, the tissue left was removed and stored in PBS. SMCs were released from the artery by trituration. Then Total RNA was extracted and gene chip tests were performed.

2016-06-30 | E-GEOD-61212 | biostudies-arrayexpress

Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

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Gene expression profiling and functional analysis in cerebral artery after experimental subarachnoid hemorrhage

Project description:The molecular mechanisms of cerebral vasopasm after SAH are not totally understood. In the present study, we analyzed gene expression profile in rabbit basilar artery after SAH using cDNA microarrays. Total RNA was extracted from rabbit basilar arteries (12 samples: day0 n=3, day3 n=3, day5 n=3, and day7 n=3). They were analyzed by microarray.

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High-throughput RNA sequencing of basilar artery smooth muscle cells in wild type and high-salt diet induced hypertension rats

Project description:We utilized high-throughput sequencing and subsequent signaling pathway analyses to find 2 fold change or greater upregulated expression of 230 transcripts and downregulated expression of 165 transcripts in basilar artery smooth muscle cells derived from rats fed a high-salt diet compared with those from control rats.

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Oryctolagus cuniculus

Project description:Microarray-based gene expression of rabbit basilar artery smooth muscle cells exposed to hemodynamic insult

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Gene expression profiling and functional analysis in cerebral artery after experimental subarachnoid hemorrhage

2013-03-06 | GSE44910 | GEO

Transcriptional profiling of basilar artery and basilar terminus intima and media in rabbits after bilateral carotid ligation

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Expression data from adult mouse mesenteric arteries

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2013-02-26 | E-GEOD-44663 | biostudies-arrayexpress

Changes in miR expression profile carotid plaques induced by acutely induced flow disturbances in apoe-/- mice on a high fat diet.

Project description:Atherosclerosis is causally related to disturbed flow through low and oscillatory shear stress. In order to study the miR expression profile in atherosclerotic plaques induced by disturbed flow, partial ligation of the carotid artery was performed. This procedure results acutely in severly reduced blood flow and in stenotic lesion formation within 6 weeks in apoe-/- mice on a high fat diet. We compared the miR expression profile in partially ligated left carotid arteries with the untreated right carotid artery to identify miRs which are involved in plaque formation through flow disturbances. The left carotid arteries of 6 female apoe-/- mice (6-8 weeks) were partially ligated (i.e. the external and internal carotid artery as well as the occipital artery were occluded; blood flow out of the common carotid artery occurs mainly through the superior thyroid artery). Following partial ligation the animals were fed a high fat diet for 6 weeks. Total RNA was isolated from partially ligated left carotid arteries and untreated right carotid arteries (control). MiRs expression profile of the partially ligated carotid arteries were compared with the control group. Biological replicates: 6 per group. One replicate per array.

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