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SMYD2 specificly regulate BIX-01294 induced TP53 target genes revealed by RNA-Seq

ABSTRACT: We characterized expression profilings of SMYD2 knockdown and control cells treated by BIX-01294 and Rapamycin.

ORGANISM(S): Homo sapiens

PROVIDER: GSE61255 | GEO | 2015/01/01

SECONDARY ACCESSION(S): PRJNA260609

REPOSITORIES: GEO

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SMYD2 specificly regulate BIX-01294 induced TP53 target genes revealed by RNA-Seq

Project description:We characterized expression profilings of SMYD2 knockdown and control cells treated by BIX-01294 and Rapamycin. We used siRNAs to knockdown SMYD2 in HCT116 cells, and NC as control. We treated those cell lines with BIX-01294 and Rapamycin, and the expression profilings were perfomed by Illumina high throughput sequencing.

2015-01-01 | E-GEOD-61255 | biostudies-arrayexpress

The G9a histone methyltransferase inhibitor BIX-01294 modulates gene expression during gametocyte development and transmission

Project description:Transmission of the malaria parasite Plasmodium falciparum from the human to the mosquito is initiated by sexual precursor cells, the gametocytes. Gametocytes are formed in response to stress signals and following up-take by a blood-feeding Anopheles mosquito initiate sexual reproduction. Gametocytes need to fine tune their gene expression in order to develop inside the mosquito to continue life-cycle progression. Previously we showed that post-translational histone acetylation controls gene expression during gametocyte development and transmission. However, the role of histone methylation remains poorly understood. We here use the histone G9a methyltransferase inhibitor BIX-01294 to investigate the role of histone methylation in regulating gene expression in gametocytes. Growth assays demonstrated that BIX-01294 inhibits intraerythrocytic replication with an IC50 of 13.0 nM. Furthermore, BIX-01294 significantly impairs gametocyte maturation and reduces the formation of gametes and zygotes. Comparative transcriptomics between BIX-01294-treated and untreated immature, mature and activated gametocytes demonstrated greater than 1.5-fold deregulation of over 359 genes. The majority of these genes are transcriptionally down-regulated in the activated gametocytes and could be assigned to transcription, translation, and signaling indicating a contribution of histone methylations in mediating gametogenesis. Our combined data shows that inhibitors of histone methylation may serve as a multi-stage antimalarials.

2019-11-25 | GSE136008 | GEO

Homo sapiens

Project description:SMYD2 specificly regulate BIX-01294 induced TP53 target genes revealed by RNA-Seq

| PRJNA260609 | ENA

Quantitative proteomics of Giardia exposures to HKMT inhibitors

Project description:We explored differential protein expression (DEPs) during sublethal exposures of HKMT inhibitors (BIX-01294 = 10uM (B), Chaetocin = 20uM (C)) in log-phase (growth; TY) and encystation (differentiation; EC) at 8 and 24 hours relative to DMSO controls (D). In total, 18,527 peptides from 1,981 proteins in log-phase, and 19,852 peptides from 2,097 proteins were analysed in encysting cultures. In total, 56 proteins were up- and 36 down-regulated in log-phase trophozoites and 32 up- and 12 down during encystation upon BIX-01294-treatment relative to the DMSO controls. Following Chaetocin-treatment, 195 proteins were up- and 121 down-regulated in log-phase trophozoites, and 63 up- and 12 down during encystation. We have presented results by cell-phase given positive correlations were driven by culture condition and not between either Chaetocin or BIX-01294 inhibitor).

2020-08-04 | PXD016747 | Pride

The effects of SMYD2 overexpression on ectopic RB in SAOS cells

Project description:Expression analysis of wild-type SAOS cells and SAOS cells transiently transfected with RB, SMYD2, or RB and SMYD2.

2011-01-01 | GSE22842 | GEO

Genome-wide maps of chromatin state of Smyd2 binding in renal epithelial cells

Project description:To identify potential transcriptional targets of Smyd2, we performed ChIP-sequencing (ChIP-seq) analysis in renal epithelial cells with an anti-Smyd2 antibody. We identified 91 potential Smyd2 target genes in Pkd1 wild type MEK cells and 116 potential Smyd2 target genes in Pkd1 null MEK cells. There were 14 genes identified in both Pkd1 wild type and null MEK cells. Thus, the number of potential Smyd2 target genes in Pkd1 null MEK cells are 116 - 14 = 102 genes

2017-08-30 | GSE97708 | GEO

The effects of SMYD2 overexpression on ectopic RB in SAOS cells

Project description:Expression analysis of wild-type SAOS cells and SAOS cells transiently transfected with RB, SMYD2, or RB and SMYD2. Four samples of wild-type, rb1, smyd2 and rb1-smyd2 were analyzed on the Human Genome U133 Plus 2.0 array.

2011-01-01 | E-GEOD-22842 | biostudies-arrayexpress

Chip-chip from MDA-MB-231 cells with SMYD2 and EZH2

Project description:The histone methyl transferase EZH2 and SMYD2 are master epigenetic regulators involved in histone methylation and gene transcription repression. Here, we report that SMYD2 and EZH2 may act as a complex in transcriptional regulation and are involved in the progression of breast cancer. ChIP-on-chip assays were performed to find unique promoters co-targeted by EZH2 and SMYD2 in MDA-MB-231 cells.

2019-11-03 | GSE126055 | GEO

Transcription profiling of human embryonic kidney cells overexpressing SMYD2 to identify which genes are regulated by SMYD2 through its histone lysine methylation activity

Project description:Identification of SMYD2 target genes - The objective of this aim is to identify which genes are regulated by SMYD2 through its histone lysine methylation activity. It is well established that post-translational modification of nucleosomal histones (H2A, H2B, H3 and H4), such as methylation, acetylation and phosphorylation are central to the proper control of gene expression

2008-02-05 | E-MEXP-963 | biostudies-arrayexpress

RNA-seq of human HT-29 WT and SMYD2-KO xenograft implanted into the flanks of Rag1−/− mice

Project description:Our data suggested that SMYD2 deficiency sensitized cells to apoptosis and necroptosis.To further narrow down the mechanism of cell death induction upon SMYD2 deficiency, RNA-seq analysis was performed on implanted tumor tissues from two clones of SMYD2 deficient and WT HT-29 cells.

2021-10-18 | E-MTAB-11053 | biostudies-arrayexpress

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