Dataset Information

The role of Cdpk6 in development

ABSTRACT: The research programme is focussed on determining the role of CDPKs in abiotic stress response. We chose several CDPKs for investigation, some which have been implicated in the response to abiotic stress (Sheen 1996), others whose function is unknown. We constructed gene fusions with YFP as a 3_in-frame fusion to investigate cellular/tissue expression; the gene-specific DNA includes 1.5kb 5'-untranslated region. Plants carrying the Cdpk6-yfp construct show a marked alteration in phenotype: the (probable)-heterozygote is small and bushy and the (probable)-homozygote shows an excess of flower primordia and insufficient leaf development. These plants do not survive. The expression of Cdpk6 as measured using a GUS-promoter fusion is confined to the developing flowerheads although the Cdpk6-YFP plants show more widespread expression. We have made RNA from two separate insertion lines of Cdpk6-YFP and from Col-0 plants carrying the aequorin gene all grown on selective medium. We cannot collect homozygote seed; the seedlings germinating from seeds collected from heterzygous plants grown on selective medium will consist of both heterozygous and homozygous individuals. Keywords: strain_or_line_design

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE6147 | GEO | 2007/01/08

SECONDARY ACCESSION(S): PRJNA97685

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:S23 experiment: We sought to identify the microRNAs (miRNAs) enriched in the neural crest cell (NCC) population from E11.5 mouse embryos. To accomplish this, we utilized a transgenic mouse line harboring Cre-recombinase under the control of the Wnt1 NCC-specific promoter and also carrying the R26R-YFP allele. We sorted YFP+ (NCCs) and YFP- (non-NCCs) from E11.5 Wnt1Cre-R26R mouse embryos via FACS and compared the relative enrichment of miRNAs in the YFP+ population by miRNA microarray. 220 experiment: We sought to identify the microRNAs (miRNAs) enriched in the neural crest cell (NCC) population from E10.5 mouse embryos. To accomplish this, we utilized a transgenic mouse line harboring Cre-recombinase under the control of the Wnt1 NCC-specific promoter and also carrying the R26R-YFP allele. We sorted YFP+ (NCCs) and YFP- (non-NCCs) from E10.5 Wnt1Cre-R26R mouse embryos via FACS and compared the relative enrichment of miRNAs in the YFP+ population by miRNA microarray. 221 experiment: Our research has shown that heterozygous deletion of the miRNA processing enzyme Dicer leads to developmental delay of the thymus in mouse embryos. We sought to identify the microRNAs (miRNAs) affected by the loss of a single copy of Dicer in the neural crest cell (NCC) population from E10.5 mouse embryos. To accomplish this, we utilized a transgenic mouse line harboring a floxed allele of Dicer, Cre-recombinase under the control of the Wnt1 NCC-specific promoter, and also carrying the R26R-YFP allele. We sorted YFP+ (NCCs) cells from E10.5 Dicerfl/+,Wnt1Cre,R26R and Dicer+/+,Wnt1Cre,R26R mouse embryos via FACS and compared the relative expression of miRNAs in the Dicer-heterozygotes compared to Dicer-wildtypes by miRNA microarray. S23 experiment: RNA from YFP+ (NCCs) and YFP- (non-NCCs) cells sorted by FACS from E11.5 Wnt1Cre-R26R mouse embryos was isolated and hybridized to Exiqon miRNA microarrays v10. Cells from five E11.5 embryos were sorted into YFP+ and YFP- populations and pooled. 220 experiment: RNA from YFP+ (NCCs) and YFP- (non-NCCs) cells sorted by FACS from E10.5 Wnt1Cre-R26R mouse embryos was isolated and hybridized to Exiqon miRNA microarrays v10. Cells from ten E10.5 embryos were sorted into YFP+ and YFP- populations and pooled. 221 experiment: RNA from YFP+ (NCCs) cells sorted by FACS from E10.5 Dicerfl/+,Wnt1Cre,R26R and Dicer+/+,Wnt1Cre,R26R mouse embryos was isolated and hybridized to Exiqon miRNA microarrays v10. Cells from ten embryos were sorted into YFP+ populations and pooled for each genotype.

Dataset Information

The role of Cdpk6 in development

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets