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The transcriptome of muscle and liver is responding differently to a combined trenbolone acetate and estradiol implant in cattle

ABSTRACT: In the present study, an attempt to compare bovine transcriptome of two different tissue matrices (liver and muscle) treated with the same molecules (trenbolone acetate (TBA) and estradiol (E2)) was carried out in order to understand the response of different tissues to the same treatment on a genomic/transcriptomic level. The primary aim of this study was to compare, via transcriptomic approach, the response of both muscle and liver to anabolic steroids, and to point out the main differences and similarities between both matrices. In addition, a second aim was to list out the main differentially expressed (DE) genes that can be used as a “pool” for biomarker investigations in the future. A bovine oligo microarray platform (GPL7053) was used to profile gene expression from thirty (30) bovine liver and muscle (biceps brachii) samples. Isolated and purified total RNAs were individually hybridized to the Agilent bovine V1 4x44k DNA microarray. The comparison of untreated and treated bovine transcriptomes revealed a set of differentially expressed genes. After functional analysis and qPCR validation, the result showed that the two transcriptomes showed completely different patterns, in terms of altered pathways and involved biological processes, following the treatment with anabolic steroids. However, they had 20 commonly expressed genes in-between.

ORGANISM(S): Bos taurus

PROVIDER: GSE62002 | GEO | 2015/10/02

SECONDARY ACCESSION(S): PRJNA262912

REPOSITORIES: GEO

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Project description:20-Hydroxyecdysone (20E) is the most abundant phytoecdysteroid produced by several plant families and has been attributed with numerous pharmacological properties in animals including anabolic and immune modulating effects. However, the in vivo bioactivity of phytoecdysteroids in mammals remains ambiguous, and moreover, the mechanism of action in mammals has yet to be determined. In this study, the physiological and gene expression effects of 20E were analyzed in C57BL/6 mice given a continuous infusion of saline or 20E (5 mg/kg/day) for five or fifteen days using subcutaneously implanted Alzet® osmotic pumps. The weights of the total body, muscle groups and organs were recorded and carcass composition was determined to evaluate changes in nutrient partitioning. There was a significant increase (p = 0.01) in the weight of triceps brachii in mice treated with 20E for five days (115 +/- 8 mg) compared to mice treated with saline for five days (88 +/- 3 mg), however, there were no differences in the other measured parameters. To determine potential mechanisms of action of 20E in skeletal muscle, Illumina’s Mouse Whole Genome-6 v2.0 Expression BeadChips were used to evaluate changes in gene expression after 20E infusion. False Discovery Rate correction of microarray data did not reveal any beadtypes that were differentially expressed. Therefore, Ingenuity Pathways Analysis (IPA) was used to identify genes with the most evidence for differential expression in the context of biological functions and pathways. IPA provided evidence for 20E involvement in processes including cellular growth and proliferation and cell-to-cell signaling and interaction. Overall, the data suggests that 20E does not have potent anabolic properties. IPA identified potential lead biological processes and pathways of 20E. Total RNA was extracted from the triceps brachii of C57BL/6 mice given a continuous infusion of saline or 20E (5 mg/kg/day) for 5 or 15 days using subcutaneously implanted Alzet® osmotic pumps to evaluate gene expression effects of 20E on mouse muscle.

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The transcriptome of muscle and liver is responding differently to a combined trenbolone acetate and estradiol implant in cattle

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