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Gene expression in E9.5 heads comparing wt with Arid3b-null embryos

ABSTRACT: We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 heads, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.

ORGANISM(S): Mus musculus

PROVIDER: GSE62070 | GEO | 2014/10/07

SECONDARY ACCESSION(S): PRJNA263157

REPOSITORIES: GEO

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Gene expression in E9.5 heads comparing wt with Arid3b-null embryos

Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 heads, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Heads were dissected from E9.5 embryos (including head and first branchial arch). Four wild-type and four mutant embryos were collected and pooled for each of the microarray triplicates.

2014-10-07 | E-GEOD-62070 | biostudies-arrayexpress

Gene expression in E9.5 trunks comparing wt with Arid3b-null embryos

2014-10-07 | GSE62071 | GEO

Gene expression in E9.5 hearts comparing wt with Arid3b-null embryos

2014-10-07 | GSE62069 | GEO

Gene expression in E9.5 hearts comparing wt with Arid3b-null embryos

Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 hearts, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Hearts were dissected from E9.5 embryos (including the heart tube and the pharyngeal mesoderm and endoderm located beneath). Four wild-type and four mutant embryos were collected and pooled for each of the microarray triplicates.

2014-10-07 | E-GEOD-62069 | biostudies-arrayexpress

Gene expression in E9.5 trunks comparing wt with Arid3b-null embryos

Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 trunks, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Trunks were dissected from E9.5 embryos (including the body from the otic vesicle to somite 15 and forelimbs). Four wild-type and four mutant embryos were collected and pooled for each of the microarray triplicates.

2014-10-07 | E-GEOD-62071 | biostudies-arrayexpress

Gene expression in whole mouse E9.0 embryos comparing wt with Arid3b-null embryos

Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b at E9.0 days of development, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.

2014-10-07 | GSE62068 | GEO

Gene expression in whole mouse E9.0 embryos comparing wt with Arid3b-null embryos

2014-10-07 | E-GEOD-62068 | biostudies-arrayexpress

Mus musculus

Project description:Gene expression in E9.5 heads comparing wt with Arid3b-null embryos

| PRJNA263157 | ENA

Gene expression at E9.5 after deletion of type I BMP receptors in the mouse lens placode

Project description:The type I BMP receptors, Bmpr1a and Acvr1 were conditionally deleted from the mouse lens placode with Le-Cre. The heads of wild type and knockout E9.5 mouse embryos were laser micodissected to isolate the lens ectoderm from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN Ovation Pico WTA system kit. cDNA was biotinylated and hybridized to Illumina Mouse6 V1.1 bead arrays.

2015-03-09 | GSE64005 | GEO

Genome-wide characterization of ARID3B binding sites and KDM4C in ARID3B-knockout OECM1 cells

Project description:In this study we assayed for genome-wide localization of ARID3B and KDM4C enrichment in control and ARID3B-knockout OECM1 cells. The expression of the embryonic stem cell (ESC) signature in cancer cells indicates the coordinated regulation of the stemness genes in cancer stem cells, which are responsible for cancer initiation and dissemination. let-7 family microRNAs are crucial regulators for stem cell differentiation. In cancer cells, let-7 suppresses cancer stemness through targeting different oncogenes such as c-Myc, RAS, and HMGA2. However, most let-7 target genes are oncogenes rather than stemness factors, and the mechanism of let-7-repressed stemness is unclear. Here we demonstrate that let-7 supresses the formation of AT-rich interacting domain 3B (ARID3B) complex through targeting the expression of ARID3B, the interacting partner ARID3A, and importin 9. ARID3B complex recruits histone demethylase 4C (KDM4C) to the regulatory region of stemness genes for reducing histone 3 lysine 9 trimethylation, resulting in an open configuration of the chromatin of stemness genes. In cancer tissues, ARID3B expression correlates with the nuclear ARID3A expression and a worse prognosis. This result highlights the role of let-7 in regulating stemness through histone modifications.

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