Genomics

Dataset Information

0

Upstream Anti-sense Promoters Act as Local Enhancers of Mammalian Protein-coding Genes


ABSTRACT: Anti-sense transcription originating upstream of mammalian protein-coding genes is a well-documented phenomenon, but remarkably little is known about the function or regulation of these anti-sense promoters or the non-coding RNAs they generate. Here we define at nucleotide resolution the divergent transcription start sites (TSSs) near mouse mRNAs. We find that coupled sense and anti-sense TSSs form the boundaries of an evolutionarily conserved and nucleosome-depleted regulatory region with dramatically enriched transcription factor (TF) occupancy. Notably, as the distance between sense and anti-sense TSSs increases, so does the level of TF binding and signal-dependent gene activation. We further discover a cluster of anti-sense TSSs in macrophages with an enhancer-like chromatin signature. Remarkably, this signature identifies promoters that are selectively and rapidly activated during immune challenge. We conclude that anti-sense TSSs can serve as potent, local enhancers of sense-strand gene expression by facilitating TF binding and deposition of activating histone modifications.

ORGANISM(S): Mus musculus

PROVIDER: GSE62151 | GEO | 2015/06/23

SECONDARY ACCESSION(S): PRJNA263290

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-06-23 | E-GEOD-62151 | biostudies-arrayexpress
| PRJNA725532 | ENA
2023-08-28 | GSE239925 | GEO
| PRJNA725536 | ENA
| PRJNA725535 | ENA
2022-05-02 | GSE173443 | GEO
2022-05-02 | GSE173442 | GEO
2012-09-05 | E-MTAB-1250 | biostudies-arrayexpress
| PRJNA263290 | ENA
2014-11-10 | GSE60455 | GEO