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Expression analysis of miR-302 knockout and wild-type embryos during neurulation

ABSTRACT: Wild-type or miR-302 knockout tissue was isolated from embryos during neurulation in replicate

ORGANISM(S): Mus musculus

PROVIDER: GSE62396 | GEO | 2015/08/03

SECONDARY ACCESSION(S): PRJNA264011

REPOSITORIES: GEO

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Expression analysis of miR-302 knockout and wild-type embryos during neurulation

Project description:Wild-type or miR-302 knockout tissue was isolated from embryos during neurulation in replicate Performed in biological triplicate except for E7.5 WT which was in duplicate and E9.5 timepoints which were in quadruplicate. Biological replicate samples represent independent embryos. Total RNA collected with Trizol. All processing conducted at the UCLA Neuroscience Genomics Core. MouseRef-8 v2.0 Expression BeadChips.

2015-08-03 | E-GEOD-62396 | biostudies-arrayexpress

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miR-302-367 regulates cardiomycyte proliferation and differentiation during development

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Expression data of reprogrammed miR-302/367-iPS cells

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Expression data of reprogrammed miR-302/367-iPS cells

Project description:Pluripotent marker correlation between miR-302/367-iPS, ES and fibroblast cells Mouse fibroblast were reprogrammed with miR-302/367 lentiviral vector, total RNA was extracted by trizol and microarray assay was performed

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MiR-302/367 regulate neural progenitor proliferation, differentiation timing, and survival in neurulation.

Project description:How neural progenitor cell (NPC) behaviors are temporally controlled in early developing embryos remains undefined. The in vivo functions of microRNAs (miRNAs) in early mammalian development remain largely unknown. Mir-302/367 is a miRNA cluster that encodes miR-367 and four miR-302 members (miR302a-d). We show that miR-302b is highly expressed in early neuroepithelium and its expression decline as development progresses. We generated a mir-302/367 knockout mouse model and found that deletion of mir-302/367 results in an early embryonic lethality and open neural tube defect (NTD). NPCs exhibit enhanced proliferation, precocious differentiation, and decreased cell survival in mutant embryos. Furthermore, we identified Fgf15, Cyclin D1, and D2 as direct targets of miR-302 in NPCs in vivo, and their expression is enhanced in mutant NPCs. Ectopic expression of Cyclin D1 and D2 increases NPC proliferation, while FGF19 (human ortholog of Fgf15) overexpression leads to an increase of NPC differentiation. Thus, these findings reveal essential roles of miR-302/367 in orchestrating gene expression and NPC behaviors in neurulation; they also point to miRNAs as critical genetic components associated with neural tube formation.

| S-EPMC4688151 | biostudies-literature

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