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Global gene expression analysis in paired fresh-frozen and FFPE furan-exposed mouse liver samples

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE62843 | GEO | 2015/10/14

SECONDARY ACCESSION(S): PRJNA265825

REPOSITORIES: GEO

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Global gene expression analysis of fresh-frozen, furan-exposed mouse liver using one-colour Agilent microarrays

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Project description:Furan is a mouse and rat hepatocarcinogen. We sought to determine if furan-induced gene expression changes could be detected in paired fresh-frozen (GSE48644) and formalin-fixed paraffin embedded (FFPE; this study) samples using two-colour microarrays. To determine the effect of time-in-formalin on gene expression signatures we performed microarray analysis of livers that were fixed in formalin for 18 hours or 3 weeks. All samples (fresh-frozen, 18 hours in formalin, 3 weeks in formalin) were also examined using one-colour microarrays and RNA-seq (ribo-depletion and polyA-enrichment protocols) in order to determine the effect of the technology on gene expression profiles.

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