Doxorubicin induces double-strand breaks at CpG island promoters
Ontology highlight
ABSTRACT: Doxorubicin is a widely used chemotherapeutic drug that intercalates between DNA base-pairs and posions Topoisomerase II, although the mechanistic basis for cell killing remains speculative. Here we show that both anthracyclines and Topoisomerase II poison cause enhanced DNA double-strand breaks around CpG island promoters of active genes genome-wide. We propose that torsion-based enhancement of nucleosome turnover exposes promoter DNA, ultimately causing DNA breaks around promoters that contributes to cell killing.
ORGANISM(S): Mus musculus Saccharomyces cerevisiae
PROVIDER: GSE62927 | GEO | 2015/01/31
SECONDARY ACCESSION(S): PRJNA266225
REPOSITORIES: GEO
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