Genomics

Dataset Information

0

The PRC2-associated factor C17orf96 is a novel CpG island regulator in mouse ES cells


ABSTRACT: CpG islands (CGIs) are key DNA regulatory elements in the vertebrate genome and are often found at gene promoters. In mammalian embryonic stem (ES) cells, CGIs are decorated by either the active or repressive histone marks, H3K4me3 and H3K27me3, respectively, or by both modifications (‘bivalent domains’), but their precise regulation is incompletely understood. Remarkably, we find that the polycomb repressive complex 2 (PRC2)-associated protein C17orf96 (a.k.a. esPRC2p48 and E130012A19Rik) is present at most CGIs in mouse ES cells. At PRC2-rich CGIs, loss of C17orf96 results in an increased chromatin binding of Suz12 and elevated H3K27me3 levels concomitant with gene repression. In contrast, at PRC2-poor CGIs, located at actively transcribed genes, C17orf96 colocalizes with RNA polymerase II and its depletion leads to a focusing of H3K4me3 in the core of CGIs. Our findings thus identify C17orf96 as a novel context-dependent CGI regulator.

ORGANISM(S): Mus musculus

PROVIDER: GSE63491 | GEO | 2015/04/29

SECONDARY ACCESSION(S): PRJNA268027

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-04-29 | E-GEOD-63491 | biostudies-arrayexpress
2016-11-17 | GSE76536 | GEO
2016-11-17 | GSE79318 | GEO
2016-11-17 | GSE85681 | GEO
2016-11-21 | GSE79606 | GEO
2023-02-22 | MSV000091349 | MassIVE
2019-09-09 | PXD009794 | Pride
2021-05-13 | PXD016781 | Pride
2012-11-01 | E-GEOD-36905 | biostudies-arrayexpress
2021-01-25 | GSE146800 | GEO