Transcriptomics

Dataset Information

0

Aging-associated inflammatory and oxidative changes in the rat urinary bladder and dorsal root ganglia - preventive effect of caloric restriction [bladder]


ABSTRACT: Purpose: To disclose the molecular mechanisms of aging-related functional impairments of the rat bladder, and to determine whether long-term caloric restriction (CR) may have preventive effects on these mechanisms. Materials and Methods: Male Fischer 344 rats were divided into three groups: young (6 months-old) fed ad libitum (Y, N = 16), old (25-28 months-old) fed ad libitum (O+AL, N = 15), and old (25-28 months-old) fed restrictedly three days a week since 6 weeks-old (O+CR, N = 16). cDNA microarray analysis and real-time polymerase chain reaction (RT-PCR) of the bladder and L6 dorsal root ganglia (L6DRG) were performed. The bladder was subjected to oxidative stress measurement and immunohistochemistry. Results: In the O+AL group, 83 genes in the bladder and 48 genes in the L6DRG were up-regulated than compared with those of the Y and O+CR groups (fold change > 2). These genes were mostly related to immune- and inflammatory-responses. Immunohistochemistry showed that Granzyme B, a cytotoxic T-lymphocyte-associated serine protease, and matrix metalloproteinase 13 (Mmp13), an interstitial collagenase, were more strongly expressed in the bladder of the O+AL than the Y and O+CR groups. The level of Malondialdehyde (MDA), an oxidative stress marker, was higher in the O+AL than the Y group, whereas there were no significant differences between the O+CR and Y groups. Conclusions: In the rat, aging is associated with up-regulation of immune and inflammatory genes in the bladder and DRG as well as with oxidative and fibrotic changes of the bladder. Long-time CR reduced these aging-related changes.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE63650 | GEO | 2016/08/31

SECONDARY ACCESSION(S): PRJNA268572

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-08-31 | GSE63651 | GEO
2021-01-30 | PXD018455 | JPOST Repository
2010-05-26 | E-GEOD-7502 | biostudies-arrayexpress
2007-12-28 | GSE8418 | GEO
2007-12-28 | GSE8419 | GEO
2009-06-22 | GSE8409 | GEO
2007-12-28 | GSE8411 | GEO
2007-12-28 | GSE8414 | GEO
2007-12-28 | GSE8417 | GEO
2007-12-28 | GSE8415 | GEO