Genomics

Dataset Information

0

Chromatin-wide profiling of the protein kinase DYRK1A reveals a role as a transcriptional activator of cell growth related genes


ABSTRACT: DYRK1A is a dosage-sensitive protein kinase that fulfills key roles during development and in tissue homeostasis, and its dysregulation results in human pathologies. DYRK1A is present in both the nucleus and cytoplasm of mammalian cells, although its nuclear function remains unclear. Genome-wide analysis of DYRK1A-associated loci reveals that the kinase is recruited preferentially to promoters of genes actively transcribed by RNA polymerase II (RNAPII), which are functionally associated with translation, RNA processing and cell cycle. DYRK1A-bound promoter sequences are highly enriched in a conserved palindromic motif, which is necessary to drive DYRK1A-dependent transcriptional activation. DYRK1A phosphorylates the carboxy-terminal domain (CTD) of RNAPII at Ser2 and Ser5. Depletion of DYRK1A results in reduced association of RNAPII at the target promoters as well as hypophosphorylation of the CTD of RNAPII along the target gene bodies. Accordingly, we propose that DYRK1A acts as a transcriptional regulator by acting as a novel CTD kinase.

ORGANISM(S): Homo sapiens

PROVIDER: GSE63712 | GEO | 2015/03/11

SECONDARY ACCESSION(S): PRJNA268845

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-03-11 | E-GEOD-63712 | biostudies-arrayexpress
2023-08-01 | GSE155808 | GEO
2023-08-01 | GSE155807 | GEO
2014-03-24 | GSE55402 | GEO
2023-08-01 | GSE158714 | GEO
2024-05-22 | PXD022966 | Pride
2019-03-06 | GSE127707 | GEO
2014-03-24 | E-GEOD-55402 | biostudies-arrayexpress
2024-09-19 | PXD049700 | Pride
2011-09-01 | GSE27687 | GEO