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MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation [adipocyte]

ABSTRACT: We identified microRNA-188 differentially expressed in the BMSCs of aged and young mice and influenced on BMSCs differentiation with ageing. Then we used microarrays to detail the adipogenic gene expression during adipogenic differention of BMSCs from 188KO mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE63723 | GEO | 2014/12/02

SECONDARY ACCESSION(S): PRJNA268914

REPOSITORIES: GEO

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MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation [osteoblast]

Project description:We identified microRNA-188 differentially expressed in the BMSCs of aged and young mice and influented on BMSCs differentiation with ageing. Then we used microarrays to detail the osteogenic gene expression during osteogenic differention of BMSCs from 188KO mice.

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MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation

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MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation.

Project description:Bone marrow mesenchymal stem cells (BMSCs) exhibit an age-dependent reduction in osteogenesis that is accompanied by an increased propensity toward adipocyte differentiation. This switch increases adipocyte numbers and decreases the number of osteoblasts, contributing to age-related bone loss. Here, we found that the level of microRNA-188 (miR-188) is markedly higher in BMSCs from aged compared with young mice and humans. Compared with control mice, animals lacking miR-188 showed a substantial reduction of age-associated bone loss and fat accumulation in bone marrow. Conversely, mice with transgenic overexpression of miR-188 in osterix+ osteoprogenitors had greater age-associated bone loss and fat accumulation in bone marrow relative to WT mice. Moreover, using an aptamer delivery system, we found that BMSC-specific overexpression of miR-188 in mice reduced bone formation and increased bone marrow fat accumulation. We identified histone deacetylase 9 (HDAC9) and RPTOR-independent companion of MTOR complex 2 (RICTOR) as the direct targets of miR-188. Notably, BMSC-specific inhibition of miR-188 by intra-bone marrow injection of aptamer-antagomiR-188 increased bone formation and decreased bone marrow fat accumulation in aged mice. Together, our results indicate that miR-188 is a key regulator of the age-related switch between osteogenesis and adipogenesis of BMSCs and may represent a potential therapeutic target for age-related bone loss.

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MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

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CGRP Regulates the Age-Related Switch Between Osteoblast and Adipocyte Differentiation.

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Escherichia coli strain:188 | isolate:188

Project description:Escherichia coli strain:188 | isolate:188 Genome sequencing

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Vibrio splendidus strain:188-18 | isolate:188-18

Project description:Vibrio splendidus strain:188-18 | isolate:188-18 Genome sequencing

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Vibrio lentus strain:188-14 | isolate:188-14

Project description:Vibrio lentus strain:188-14 | isolate:188-14 Genome sequencing

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High-throughput sequencing identified differentially expressed RNAs between MALAT1 knockdown C3H10 adipocyte and control cells

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