High-throughput small RNA sequencing of mouse primary macrophages treated with M-CSF withdrawal
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE63801 | GEO | 2015/10/15
SECONDARY ACCESSION(S): PRJNA269108
REPOSITORIES: GEO
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Project description:The goal of this study is to investigate the presence of novel small RNAs in apoptotic macrophage Method: cultured mouse (bl6 line) bone marrow were differentiated in for 7 days in conditioned medium, in preence of Macrophage-Colony Stimulating Factor (M-CSF). M-CSF also stimulates the survive of macrophages by activating PI3K/AKT pathway. In accordance to previous publication (Lombardo, E., Alvarez-Barrientos, A., Maroto, B., Bosca, L., and Knaus, U.G, 2007, TLR4-mediated survival of macrophages is MyD88 dependent and requires TNF-alpha autocrine signalling, J Immunol 178, 3731-3739) apoptosis was induced in culturaed bone marrow-derived macrophages upon 36 hr of M-CSF withdrawal. Total RNA was isolated and a small RNA library was made using "Small RNA sequencing kit" (Illumina), according to the manufacturer’s instructions.
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