High-throughput Mapping of the Chromatin Structure of Human Promoters
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ABSTRACT: Understanding how chromatin structure affects cellular functions such as transcription and replication in human cells has been limited by a lack of sufficient nucleosome positioning data. We describe a high-resolution microarray approach combined with a novel analysis algorithm to examine the translational nucleosome positions in 3,692 promoters within seven human cell lines. Unlike unexpressed genes without transcription pre-initiation complexes at their promoters, expressed genes or genes containing pre-initiation complexes exhibit characteristic nucleosome-free regions at their transcription start sites. Coupling these data to ChIP-chip analyses reveals that the melanocyte master transcriptional regulator MITF binds predominantly to nucleosome-free regions, supporting the model that nucleosomes limit sequence accessibility. This study thus presents the first global view of human nucleosome positioning and provides a high-throughput tool for analyzing chromatin structure in development and disease. Keywords: Nucleosome positions, MNase digestion, cell type comparison
ORGANISM(S): Homo sapiens
PROVIDER: GSE6385 | GEO | 2007/01/15
SECONDARY ACCESSION(S): PRJNA99559
REPOSITORIES: GEO
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