Other

Dataset Information

0

HuR- dependent regulation of mRNA splicing is essential for the B cell antibody response [iCLIP-Seq]


ABSTRACT: Post-transcriptional regulation of mRNA by the RNA binding protein HuR is required in B cells for the germinal centre reaction and for the production of class-switched antibodies in response to T-independent antigens. Transcriptome-wide examination of RNA isoforms, abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interaction, revealed that HuR-dependent mRNA splicing affects hundreds of transcripts including the dihydrolipoyl succinyltransferase (Dlst), a subunit of the aketoglutaratedehydrogenase (aKGDH) enzyme. In the absence of HuR, defective mitochondrial metabolism results in high levels of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for B cell proliferation and differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE63905 | GEO | 2015/02/13

SECONDARY ACCESSION(S): PRJNA269380

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-02-13 | GSE62134 | GEO
2015-02-13 | GSE62129 | GEO
2015-02-13 | E-GEOD-62129 | biostudies-arrayexpress
2015-02-13 | E-GEOD-62134 | biostudies-arrayexpress
2013-11-01 | E-GEOD-49225 | biostudies-arrayexpress
2021-09-22 | GSE145413 | GEO
2013-11-01 | GSE49225 | GEO
2011-12-31 | GSE33055 | GEO
2011-12-31 | E-GEOD-33055 | biostudies-arrayexpress
2011-06-29 | E-GEOD-29943 | biostudies-arrayexpress