HuR- dependent regulation of mRNA splicing is essential for the B cell antibody response [iCLIP-Seq]
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ABSTRACT: Post-transcriptional regulation of mRNA by the RNA binding protein HuR is required in B cells for the germinal centre reaction and for the production of class-switched antibodies in response to T-independent antigens. Transcriptome-wide examination of RNA isoforms, abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interaction, revealed that HuR-dependent mRNA splicing affects hundreds of transcripts including the dihydrolipoyl succinyltransferase (Dlst), a subunit of the aketoglutaratedehydrogenase (aKGDH) enzyme. In the absence of HuR, defective mitochondrial metabolism results in high levels of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for B cell proliferation and differentiation.
ORGANISM(S): Mus musculus
PROVIDER: GSE63905 | GEO | 2015/02/13
SECONDARY ACCESSION(S): PRJNA269380
REPOSITORIES: GEO
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