Transcriptomics

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Ex vivo mouse model for hepatic schistosomiasis


ABSTRACT: An ex vivo approach to studying hepatic schistosomiasis used thick naive mouse liver sections and sterile culturing. This tissue is unfixed, unfrozen, and alive for subsequent in vitro studies. We have cultured and monitored these sections for up to 48 hrs noting unchanged gross hepato-cellular morphology, and no significant increases in hepatotoxicity as demonstrated by media liver enzyme concentrations. Liver slices were treated with soluble egg antigen from Schistosoma japonicum eggs and cultured for 48hrs. Transcriptional changes were then analysed by microarray.

ORGANISM(S): Mus musculus

PROVIDER: GSE64071 | GEO | 2014/12/12

SECONDARY ACCESSION(S): PRJNA270033

REPOSITORIES: GEO

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