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Impact of ulaR2 delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of ascorbic acid

ABSTRACT: Transcriptome comparison of the Streptococcus pneumoniae D39 ΔulaR2 to D39 wild-type grown in M17 medium + 10mM ascorbic acid (AM17.

ORGANISM(S): Streptococcus pneumoniae D39

PROVIDER: GSE64106 | GEO | 2015/01/21

SECONDARY ACCESSION(S): PRJNA270171

REPOSITORIES: GEO

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Impact of ulaR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of ascorbic acid

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ∆ulaR to D39 wild-type grown in M17 medium + 10mM ascorbic acid (AM17)

2014-10-28 | GSE61649 | GEO

Transcriptional response of Streptococcus pneumoniae D39 to ascorbic acid

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium to grown in M17 medium + 10mM ascorbic acid (AM17).

2015-01-21 | GSE64105 | GEO

Comparison of the Streptococcus pneumoniae D39 ulaR Mutant compared to D39 wild-type

Project description:Comparison of the Streptococcus pneumoniae D39 ulaR mutant compared to D39 wild type in M17 medium + 10mM ascorbic acid (AM17) One condition design comparision of two strains including a dye swap

2014-10-28 | E-GEOD-61649 | biostudies-arrayexpress

Comparison of the Streptococcus pneumoniae D39 ulaR2 Mutant compared to D39 wild-type

Project description:In this study, we have explored the impact of ascorbic acid on the transcriptome of Streptococcus pneumoniae D39. The expression of several genes and operons, including the ula operon (which has been previously shown to be involved in ascorbic acid utilization), the AdcR regulon (which has been previously shown to be involved in zinc transport and virulence) and a PTS operon (which we denote here as ula2 operon) were altered in the presence of ascorbic acid. The ula2 operon consists of five genes, including the transcriptional activator ulaR2. Our M-NM-2-galactosidase assay data and transcriptome comparison of the ulaR2 mutant with the wild-type demonstrated that the transcriptional activator UlaR2 in the presence of ascorbic acid activates the expression of the ula2 operon. We further predict a 16-bp regulatory site (5M-bM-^@M-^Y-ATATTGTGCTCAAATA-3M-bM-^@M-^Y) for UlaR2 binding in the Pula2. Furthermore, we have explored the effect of ascorbic acid on the expression of the AdcR regulon. Our ICP-MS analysis showed that addition of ascorbic acid to the medium causes zinc starvation in the cell that leads to the activation of the AdcR regulon. Comparison of the Streptococcus pneumoniae D39 ulaR2 mutant compared to D39 wild type in M17 medium+ 10mM ascorbic acid (AM17) One condition design comparision of two strains including a dye swap

2015-01-21 | E-GEOD-64106 | biostudies-arrayexpress

Impact of bguR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔbguR to D39 wild-type grown in M17 medium + 0.5% glucose (GM17).

2013-02-20 | GSE43342 | GEO

Impact of ccpA delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔccpA to D39 wild-type grown in M17 medium + 0.5% glucose (GM17).

2015-03-06 | GSE66558 | GEO

Impact of rokA delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔrokA to D39 wild-type grown in M17 medium + 0.5% glucose (GM17).

2012-10-22 | GSE41448 | GEO

Impact of celR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Cellobiose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔcelR to D39 wild-type grown M17 medium + 0.5 % (w/v) Cellobiose (CM17).

2011-07-25 | GSE30891 | GEO

Impact of ccpA delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Maltose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔccpA to D39 wild-type grown in MM17 (0.5 % (w/v) Maltose + M17) medium

2015-04-23 | GSE65547 | GEO

Impact of lacR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔlacR to D39 wild-type grown in M17 medium + 0.5 % (w/v) Glucose (GM17).

2014-06-20 | GSE58183 | GEO

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