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Gene expression profile of the NF-κB subunit p52 in Hodgkin’s lymphoma

ABSTRACT: Malignant cells of Hodgkin's lymphoma (HL) cells are characterized by constitutive activation of the canonical as well as the non-canonical NF-κB signaling cascades. Knockdown of a subunit combination corresponding to the non-canonical NF-κB dimer (p52/RelB) in the HL cell line L-1236 caused up-regulation of a set of genes that are associated with hematopoietic and lymphoid organ development. As p52 can form homodimeric complexes, which can repress transcription either alone or in association with transcriptional repressors such as HDAC1, we knocked down p52 alone to analyze its role in gene repression in HL cells. We found that the single knockdown of p52 is indeed sufficient to up-regulate an interesting set of genes that may play a role in B-cell and/or HL development.

ORGANISM(S): Homo sapiens

PROVIDER: GSE64234 | GEO | 2016/12/21

SECONDARY ACCESSION(S): PRJNA270498

REPOSITORIES: GEO

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Gene expression profiles of canonical and non-canonical NF-κB signaling pathways in Hodgkin’s lymphoma

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2016-02-18 | GSE64232 | GEO

Gene expression profiles of canonical and non-canonical NF-ÎºB signaling pathways in Hodgkinâs lymphoma

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2016-02-18 | E-GEOD-64232 | biostudies-arrayexpress

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Expression study of the Hodgkin’s lymphoma cell line L-1236 upon CRISPR Cas9-mediated knockout of lymphotoxin alpha (LTA) compared to control

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Genome wide binding sites of NF-ÎºB subunits RelA, RelB, p50, and p52 in the Hodgkin lymphoma cell line L1236

Project description:The malignant cells of Hodgkin's lymphoma are characterized by a constitutive activation of the canonical as well as the non-canonical NF-ÎºB signaling cascades. We carried out genome-wide localization and expression profiling experiments in the Hodgkin lymphoma cell line L1236 for the canonical and non-canonical NF-ÎºB pathway components p65, p50 and p52, RelB, respectively. We found that the single NF-ÎºB subunits bind to overlapping, but distinct cistromes by using consensus motifs of high similarity. ChIP-Seq analysis of 4 NF-ÎºB subunits with 2 biological replicates each

2016-02-18 | E-GEOD-63736 | biostudies-arrayexpress

Investigating the noncanonical Nfkb2 pathway regulation of gene expression in modulating dendritic cell function.

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CDK12 mediated transcriptional regulation in U2OS cells

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