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Ovarian cancer cell line RMG1: ALDEFLUOR positive vs. negative

ABSTRACT: Transcriptional profiling of ALDH1 high cancer stem like cells of ovarian cancer cell line, sorted out using ALDEFLUOR assay.

ORGANISM(S): Homo sapiens

PROVIDER: GSE64539 | GEO | 2015/04/30

SECONDARY ACCESSION(S): PRJNA271273

REPOSITORIES: GEO

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Ovarian cancer cell line RMG1: ALDEFLUOR positive vs. negative

Project description:Transcriptional profiling of ALDH1 high cancer stem like cells of ovarian cancer cell line, sorted out using ALDEFLUOR assay. Two-condition experiment, ALDEFLUOR positive vs. negative cells. Biological replicates: 1 ALDEFLUOR positive replicate, 1 negative replicate.

2015-04-30 | E-GEOD-64539 | biostudies-arrayexpress

Human colorectal cancer cell line CRC#21: ALDH-high cells vs. ALDH-low cells

2016-04-01 | GSE77149 | GEO

Characterization of aldehyde dehydrogenase 1 high ovarian cancer cells: Towards targeted stem cell therapy

Project description:Objective: The cancer stem cell (CSC) paradigm hypothesizes that successful clinical eradication of CSCs may lead to durable remission for patients with ovarian cancer. Despite mounting evidence in support of ovarian CSCs, their phenotype and clinical relevance remain unclear. We and others have found high aldehyde dehydrogenase 1 (ALDHhigh) expression in a variety of normal and malignant stem cells, and sought to better characterize ALDHhigh cells in ovarian cancer. Methods: We compared ALDHhigh to ALDHlow cells in two ovarian cancer models representing distinct subtypes: FNAR-C1 cells, derived from a spontaneous rat endometrioid carcinoma, and the human SKOV3 cell line (described as both serous and clear cell subtypes). We assessed these populations for stem cell features then analyzed expression by microarray and qPCR. Results: ALDHhigh cells displayed CSC properties, including: smaller size, quiescence, regenerating the phenotypic diversity of the cell lines in vitro, lack of contact inhibition, nonadherent growth, multi-drug resistance, and in vivo tumorigenicity. Microarray and qPCR analysis of the expression of markers reported by others to enrich for ovarian CSCs revealed that ALDHhigh cells of both models showed downregulation of CD24, but inconsistent expression of CD44, KIT and CD133. However, the following drugable targets were consistently expressed in the ALDHhigh cells from both models: mTOR signaling, her-2/neu, CD47 and FGF18 / FGFR3. Conclusions: Based on functional characterization, ALDHhigh ovarian cancer cells represent an ovarian CSC population. Differential gene expression identified drugable targets that have the potential for therapeutic efficacy against ovarian CSCs from multiple subtypes. FNAR-C1 rat ovarian cancer cells were sorted into Aldefluor high and Aldefluor low populations. Analysis was performed in triplicate. Aldefluor low cells served as the reference cells for fold-change analysis.

2016-06-02 | E-GEOD-82112 | biostudies-arrayexpress

Expression data from mouse ALDH1 positive and ALDH1 negative ovarian surface epithelium (OSE) cells

Project description:The hilum region of the mouse ovary, the transitional/junction area between OSE, mesothelium and tubal (oviductal) epithelium is identified as a previously unrecognized stem cell niche of the OSE. OSE cells with high ALDH1 activity have been predominantly detected in the hilum region by immunohistochemical staining. For additional phenotypical characterization, we used microarrays to detail the gene expression profiling between the ALDH1 positive and ALDH1 negative OSE cells. Three independent pools (10 mice each) were analyzed. ALDH1 positive and ALDH negative OSE cells were isolated by FACS and their mRNA used for hybridization onto Affymetrix Mouse Genome 430 2.0 array. Microarray data were analyzed with GeneSifter software. To identify genes significantly altered in ALDH positive population, we performed paired two group analysis with Significance Analysis of Microarrays (SAM) software and visualized with Treeview software.

2013-01-31 | E-GEOD-43897 | biostudies-arrayexpress

Expression data from mouse ALDH1 positive and ALDH1 negative ovarian surface epithelium (OSE) cells

2013-01-31 | GSE43897 | GEO

Human colorectal cancer cell line CRC#21: ALDH-high cells vs. ALDH-low cells

Project description:Transcriptional profiling of cancer stem cells (ALDH-high cells) comparing non-cancer stem cells (ALDH-low cells), sorted out using ALDEFLUOR assay. Goal was to identity cancer stem cell-specific genes. Two-condition experiment, sphere-cultured cells vs. adherent-cultured cells: 1 ALDH-high replicate and 1 ALDH-low replicate.

2016-04-01 | E-GEOD-77149 | biostudies-arrayexpress

Virant-klun _SSEA-4-positive Cells Isolated from Adult Human Ovarian Cell Cultures

Project description:Comparison of gene expression profile of different types of cells: hESCs (H1), human adult dermal fibroblasts, cells from adult human ovarian cell culture, FACS sorted SSEA-4 positive cells from adult human ovarian culture and cells from human ovarian cancer cell culture.

2014-05-03 | E-MEXP-3774 | biostudies-arrayexpress

Single cell RNA sequencing of DDX4 antibody positive and negative ovarian tissue cells cultured under oogonial stem cell conditions

Project description:Human ovarian tissue cells positive for DDX4 on the surface were claimed to be of oogonial stem cell nature. In order to study DDX4 antibody positive putative oogonial stem cells, ovarian tissue cells were sorted into DDX4 antibody positive and negative populations and single cell RNA-seq was performed using Smart-seq2.

2020-01-30 | E-MTAB-8403 | biostudies-arrayexpress

RNA-Seq of ALDH positive, ALDH negative and unsorted cells from patient-derived 3D in-vitro models of colon cancer

Project description:Fluorescence-activated cell sorting (FACS) was used to separate colon tumor samples into two fractions of ALDH Negative and ALDH Positive cells using the Aldefluor Assay. RNA samples were derived from FAC-sorted 3D in-vitro models of patient-derived colon tumors. Samples from unfractionated in vitro models are also included.

2017-04-30 | E-MTAB-5209 | biostudies-arrayexpress

ALDH-positive MCF-7 cells

Project description:We used an Aldefluor assay system to study the signaling pathways that regulate the frequency and maintenance of ALDH-positive cell population in MCF-7 cells as a CSC model. Through microarray analysis, we investigated a possible receptor that increases the proportion of the ALDH-positive cell population in MCF-7 cells. MCF-7 were stained with Aldefluor and ALDH-positive cells were sorted using flow cytometry. Gene expression profiles of ALDH+ cells were compared with ALDH- cells.

2014-09-23 | E-GEOD-59653 | biostudies-arrayexpress

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