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Transcriptome analysis using customized AZ microarray on tomato Flower Abscission zone of transgeneic line TAPG::antisense KD gene

ABSTRACT: To extend our previous knowledge from our gene expression studies on Flower pedicel Abscission zone using Affymetrix microarray chip we have employed whole transcriptome analysis by NGS as a discovery platform to identify and add the transcripts to pre-existing database. We designed the Customized AZ Microarray chip with the transcripts obtained from NGS (includes novel transcripts), pre existing Agilent probes, and some additional transcripts from previous databases. We developed transgenic lines by antisense silencing the genes, which are expressed in the AZ to study the functional role in abscission process. In the current stuty we silenced KD gene by antisense technology under abscisson promoter (TAPG::antsisense KD). The flower pedicel AZ tissue tissue was sampled at six time points (0, 4, 8, 12, 16 and 20 h), following flower removal, and analyzed for their gene expression profiles using the customized AZ microarray.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE64564 | GEO | 2018/01/01

SECONDARY ACCESSION(S): PRJNA271308

REPOSITORIES: GEO

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Transcriptome analysis using customized AZ microarray on tomato Flower Abscission zone of transgeneic line TAPG::antisense TPRPgene

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2010-07-03 | E-GEOD-22682 | biostudies-arrayexpress

Expression profiling of tomato pre-abscission pedicels

2012-07-20 | GSE39519 | GEO

Solanum lycopersicum

Project description:Transcriptome analysis using customized AZ microarray on tomato Flower Abscission zone of transgeneic line TAPG::antisense KD gene

| PRJNA271308 | ENA

Global gene expression analysis in the pedicel abscission zone of tomato flowers by DNA microarray

2010-07-03 | GSE22682 | GEO

Solanum lycopersicum

Project description:Transcriptome Analysis using customized AZ microarray on Flower pedicel Abscission and non abscission zones in Tomato (Solanum lycopersicum c.v New Yorker) Plants.

| PRJNA270407 | ENA

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