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Snai1 regulates cell lineage allocation and stem cell maintenance in the mouse intestinal epithelium

ABSTRACT: Conditional knockout of Snai1 in the mouse intestinal epithlium results in apoptotic loss of crypt base columnar cells and bias towards differentiation of secretory lineages. In vitro organoid cultures derived from Snail conditional knockout mice also undergo apoptosis when Snai1 is deleted. Snai1 is required for regulation of lineage choice and maintenance of CBC stem cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE65005 | GEO | 2015/02/28

SECONDARY ACCESSION(S): PRJNA272707

REPOSITORIES: GEO

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Snai1 regulates cell lineage allocation and stem cell maintenance in the mouse intestinal epithelium

Project description:Conditional knockout of Snai1 in the mouse intestinal epithlium results in apoptotic loss of crypt base columnar cells and bias towards differentiation of secretory lineages. In vitro organoid cultures derived from Snail conditional knockout mice also undergo apoptosis when Snai1 is deleted. Snai1 is required for regulation of lineage choice and maintenance of CBC stem cells. Total RNA was extracted from organoids cultured for 24 hours after treatment with 0.5ÂµM Tamoxifen derived from Villin-Cre and Villin-Cre, Snai1fl/fl mice.

2015-02-28 | E-GEOD-65005 | biostudies-arrayexpress

RNA-seq data of si-Snai1, si-Snai2, si-Snai1/2 and si-Scrambled treated mouse primary myoblasts

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RNA-seq data of si-Snai1, si-Snai2, si-Snai1/2 and si-Scrambled treated mouse primary myoblasts

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SNAI1 induced epithelial to mesenchymal transition miRNA study in time course

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SNAI1 induced epithelial to mesenchymal transition miRNA study in time course

Project description:To identify miRNAs participating in SNAI1-orchestrated regulatory pathways, we analysed time-resolved microarray data of SNAI1-induced EMT, obtained during conditional expression of SNAI1 in a “Tet-Off” MCF7-SNAI1 breast carcinoma cell model (Vetter et al, 2009). miRNA time series study for 7 times points (4h, 8h, 12h, 24h, 48h, 72h, 96h) in 3 replicates. For each time point, we compared induced and non-induced samples. Overall, we have 42 samples (21 hybridizations).

2012-01-13 | E-GEOD-35074 | biostudies-arrayexpress

Transcription factor Achaete scute-like 2 (Ascl2) controls intestinal stem cell fate

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2013-12-10 | E-GEOD-53107 | biostudies-arrayexpress

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