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Expression data from SMMC-7721 cells

ABSTRACT: We performed a genome wide transcription profile analysis to determine the expression alterations between control and the POH1 siRNAs transfected SMMC-7721 cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE65210 | GEO | 2015/01/23

SECONDARY ACCESSION(S): PRJNA273406

REPOSITORIES: GEO

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Expression data from SMMC-7721 cells

Project description:We performed a genome wide transcription profile analysis to determine the expression alterations between control and the POH1 siRNAs transfected SMMC-7721 cells The liver cancer cell line SMMC-7721 transfected with either the control or POH1 siRNAs were subjected to a genome wide transcription profile analysis through Human U133 Puls 2.0 (Affymetrix) microarray

2015-01-23 | E-GEOD-65210 | biostudies-arrayexpress

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Expression profiling of SMMC-7721 cells: stably expressing lncRNA-hPVT1 vs LV-Control

Project description:In our present study, we found that lncRNA-hPVT1 could promote HCC cell proliferation. We want to know what were the target genes of lncRNA-hPVT1. So we constructed the hPVT1-overexpressed SMMC-771 cells and observed the mRNA profile in hPVT1-overexpressed and control SMMC-771 cells. lncRNA-hPVT1 induced transcriptional changes in SMMC-7721. Two-condition experiment, control (LV-Control) vs lncRNA-hPVT1 expression (LV-hPVT1 Clone2 ). Each has 3 biological repeats.

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Gene expression signatures for miR-26a over-expression and negative control in SMMC-7721 cells

Project description:To further development of our gene expression approach to microRNA-26a over-expression, we have employed whole mRNA microarray expression profiling as a discovery platform. SMMC-7721 cells at 70–80% confluence in 6-well plates were transfected using Lipofectamine 2000 (Invitrogen). Negative control mimics or miR-26a mimics (100nM) were transfected in each well. Cell extracts were prepared 48 h after transfection, total RNA was checked for a RIN number to inspect RNA integration by Affymetrix PrimeView™ Human Gene Expression Arrays. We used microarrays to detail the global programme of gene expression underlying miR-26a over-expression and identified distinct classes of up-regulated or down-regulated genes during this process.

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Transcription profiling by array of human hepatocarcinoma SMMC-7721 cells treated with a new compound IMB5043 against untreated controls

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