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MicroRNA expression in parotid acinar cells during terminal differentiation

ABSTRACT: Terminal differentiation in parotid acini relies on sustained changes in gene expression during the first few postnatal weeks. Little is known about what drives these changes. Expression measurements along with knowledgebased network analysis was used to develop a prospective gene regulatory network that drives differentiation. We used both microRNA and mRNA expression measurements along with knowledgebased network analysis was used to develop a prospective gene regulatory network that drives differentiation.

ORGANISM(S): Mus musculus Rattus norvegicus

PROVIDER: GSE65324 | GEO | 2015/01/27

SECONDARY ACCESSION(S): PRJNA273672

REPOSITORIES: GEO

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microRNA expression in parotid acinar cells during terminal differentiation

Project description:Terminal differentiation in parotid acini relies on sustained changes in gene expression during the first few postnatal weeks. Little is known about what drives these changes. Expression measurements along with knowledgebased network analysis was used to develop a prospective gene regulatory network that drives differentiation. We used both microRNA and mRNA expression measurements along with knowledgebased network analysis was used to develop a prospective gene regulatory network that drives differentiation. laser capture microdissection was used to isolate acinar cells from the parotid at four timepoints in triplicate (E20, P5, P15, and P25). RNA was isolated, and used to measure microRNA expression.

2015-01-27 | E-GEOD-65324 | biostudies-arrayexpress

mRNA expression in parotid acinar cells during terminal differentiation

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mRNA expression in parotid acinar cells during terminal differentiation

Project description:Terminal differentiation in parotid acini relies on sustained changes in gene expression during the first few postnatal weeks. Little is known about what drives these changes. Expression measurements along with knowledgebased network analysis was used to develop a prospective gene regulatory network that drives differentiation. We used expression measurements along with knowledgebased network analysis was used to develop a prospective gene regulatory network that drives differentiation. laser capture microdissection was used to isolate acinar cells from the parotid at nine timepoints in triplicate (E18, E20, P0, P2, P5, P9, P15, P20, and P25). RNA was isolated, and applied to the affymetrix rat genome array 230.

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