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Altered integrin expression patterns revealed by microarray in matched human melanomas (mRNA)

ABSTRACT: Metastatic process is considered the predominant cause of melanoma-specific death, decreasing survival dramatically, and resulting in difficulties in the effective treatment. Large variety of molecular pathways associated with disease development and progression suggests that no individual molecular alteration is crucial in these processes per se. Our aim was to gain insight into the molecular alterations behind metastasis formation, and to determine to what extent the profiles of primary melanoma and the corresponding metastasis are similar or distinct. High-throughput gene expression profiling was performed in combination with DNA microarrays to define significantly altered genes between matched primary and metastatic melanoma cell lines and tissue samples. Invasive behavior of different cell lines was determined using Matrigel invasion assays, and invasive primary clones were cultured selectively. Real-time qRT-PCR was applied to define integrin expression profiles of cell line pairs and melanoma tissue samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE65568 | GEO | 2017/03/01

SECONDARY ACCESSION(S): PRJNA274383

REPOSITORIES: GEO

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Altered integrin expression patterns revealed by microarray in matched human melanomas (snp)

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