FBXL10 and the Protection of Polycomb-bound Genes from Hypermethylation (RNA-Seq)
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ABSTRACT: Nearly all CpG-dense promoters are occupied by the multi-domain chromosomal protein FBXL10. We show here that complete inactivation of the Fbxl10 gene leads to dense de novo methylation only of the promoters that are co-occupied by both FBXL10 and by Polycomb Repressive Complexes; this results in pervasive defects in embryonic development and death of homozygous Fbxl10 mutant embryos at midgestation. Deletion of key components of Polycomb Repressive Complexes 1 and 2 did not lead to ectopic de novo methylation. These results indicate that FBXL10 defends Polycomb-occupied promoters against ectopic de novo methylation. FBXL10 is the first reported factor whose loss leads to a gain in genomic DNA methylation.
ORGANISM(S): Mus musculus
PROVIDER: GSE65582 | GEO | 2015/04/06
SECONDARY ACCESSION(S): PRJNA274413
REPOSITORIES: GEO
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