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Gene expression analysis of leukemia-initiating cells of compound URE-/+::Msh2-/- mice

ABSTRACT: Expression profiling of FACS purified Lin-cKit+ cells from compound URE-/+::Msh2-/- mice with AML and control animals

ORGANISM(S): Mus musculus

PROVIDER: GSE65668 | GEO | 2015/09/07

SECONDARY ACCESSION(S): PRJNA274678

REPOSITORIES: GEO

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Gene expression analysis of leukemia-initiating cells of compound URE-/+::Msh2-/- mice

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Gene expression analysis of leukemia-initiating cells of URE-/+::Msh2-/- mice

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:The indicated thymic progenitor population was sorted via FACS and then loaded into a Fluidigm C1 small cell capture chip for single-cell capture, lysis, reverse transcription, and preamplification. Preamplified products were analyzed on a BioMark HD with EvaGreen chemistry. The genes analyzed were selected based on bulk RNA sequencing data and/or prior publications, including genes relevant for gamma/delta T cells and T cell progenitors. B6=C57BL/6J, Rag=B6.Rag1-/-, Tcrd=B6.Tcrd-/-, DN1d=Live/TCRd-/Lin-/CD44+/CD25-/CD24+/cKit-, DN2=Live/TCRd-/Lin-/CD44+/CD25+/cKit+, cKit-=Live/TCRd-/Lin-/CD44+/CD25-/cKit-. Lin=CD3/CD8/CD11b/CD11c/CD19/Gr-1/NK1.1/TCRb/Ter-119 Sample naming nomenclature is as follows: MouseLine_CellPopoulation_DateCaptured_CaptureChamber_CellNumberInChamber

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Gene expression analysis of leukemia-initiating cells of URE-/+::Msh2-/- mice

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