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Transcript profiling upon ethanol-induced expression of Xcv type 3- effector xopB in A. thaliana

ABSTRACT: The bacterial pathogen Xanthomonas campestris pv. vesicatoria 85-10 (Xcv) translocates about 30 type 3 effector proteins (T3Es) into pepper plants (Capsicum annuum) to suppress its immune responses. Among them is XopB which was previously shown to interfere with cell wall-bound invertase (CwInv) and sugar-mediated defence responses. Transgenic Arabidopsis thaliana lines expressing xopB under control of an ethanol inducible promotor were generated to investigate the in planta activity of this effector protein in more depth. The microarray analysis revealed that expression of the bacterial T3E XopB in A. thaliana plants causes immediate and massive reprogramming of transcription resulting in the alteration of the hormonal homeostasis and imbalances in signalling cascades. Further analyses unravelled that XopB modifies the pathogen associated molecular pattern (PAMP)-triggered calcium signature and inhibits the burst of reactive oxygen species (ROS).

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE66477 | GEO | 2015/10/01

SECONDARY ACCESSION(S): PRJNA277045

REPOSITORIES: GEO

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