Transcriptomics

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Efficient and quantitative high-throughput tRNA sequencing


ABSTRACT: Despite its biological importance, transfer RNA (tRNA) could not be adequately sequenced due to the abundant presence of post-transcriptional modifications and extensive structure that interfere with cDNA synthesis and adapter ligation. We achieve efficient and quantitative tRNA sequencing by removing base methylations using engineered demethylases and using a highly processive thermo-stable reverse transcriptase without the need for adapter ligation (DMTRT-tRNA-seq). Our method should be applicable for biological investigations of tRNA in all organisms.

ORGANISM(S): Homo sapiens

PROVIDER: GSE66550 | GEO | 2015/07/01

SECONDARY ACCESSION(S): PRJNA277309

REPOSITORIES: GEO

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