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Genome wide ChIP-seq analysis of human TOP2B occupancy in MCF7 breast cancer epithelial cells

ABSTRACT: TOP2B is involved in transcriptional initiation in response to nuclear hormone ligands and plays a role in transcriptional elongation. Whole genome TOP2B ChIP-seq was carried out on human MCF7 cells in the presence and absence of the nuclear hormone estradiol. Three peak calling methods were used and the peaks identified by at least two methods were analyzed further. Approximately half of the peaks fell either within a gene or within 5Kb of a transcription start site. The coincidence of TOP2B peaks and gene promoters was analyzed; TOP2B peaks were less frequently associated with promoters in estradiol treated than in control cells, suggesting a role of TOP2B in repression of transcription or a transient role in estradiol induced transcriptional changes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE66753 | GEO | 2015/09/21

SECONDARY ACCESSION(S): PRJNA277863

REPOSITORIES: GEO

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Genome wide ChIP-seq analysis of human TOP2B occupancy in MCF7 breast cancer epithelial cells

Project description:TOP2B is involved in transcriptional initiation in response to nuclear hormone ligands and plays a role in transcriptional elongation. Whole genome TOP2B ChIP-seq was carried out on human MCF7 cells in the presence and absence of the nuclear hormone estradiol. Three peak calling methods were used and the peaks identified by at least two methods were analyzed further. Approximately half of the peaks fell either within a gene or within 5Kb of a transcription start site. The coincidence of TOP2B peaks and gene promoters was analyzed; TOP2B peaks were less frequently associated with promoters in estradiol treated than in control cells, suggesting a role of TOP2B in repression of transcription or a transient role in estradiol induced transcriptional changes. Whole genome TOP2B ChIP-seq was carried out on human MCF7 cells in the presence (30 mins exposure) and absence of the nuclear hormone estradiol. "Input" control samples were also sequenced for background detection and comparison.

2015-09-21 | E-GEOD-66753 | biostudies-arrayexpress

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Project description:TOP2B is involved in transcriptional initiation in response to nuclear hormone ligands and plays a role in transcriptional elongation. Whole genome TOP2B ChIP-seq was carried out on human KG-1 cells in the presence and absence of the topoisomerase inhibitors mitoxantone and etoposide. Chromatin was assayed for TOP2B occupancy by peak calling with SISSRs and MACS.

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Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [fastGRO]

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TOP2B binding and enzymatic activity on promoters and introns modulates multiple oncogenes in human gliomas [ChIP-seq]

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