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The Gene Expression Profile of Peripheral Blood Mononuclear Cells from CA16-Infected Rhesus Neonates

ABSTRACT: The present work focuses on global gene expression of peripheral blood mononuclear cells in rhesus monkey infants with HFMD induced by CA16 infection at different time points. Genome-wide expression and analysis were performed by using Agilent whole genome microarrays and established bioinformatics tools. 931 signiﬁcantly differentially expressed genes associated with 5 gene ontology categories, including cell communication, cell cycle, immune system process, regulation of transcription and metabolic process were identified. Subsequently, by mapping genes related to immune system process on KEGG pathways, the predominance of inflammation mediate by chemokine and cytokine signaling pathway and interleukin signaling pathway emerged. Ultimately, co-expression genes and their networks were analysised.

ORGANISM(S): Macaca mulatta

PROVIDER: GSE66757 | GEO | 2015/03/10

SECONDARY ACCESSION(S): PRJNA277870

REPOSITORIES: GEO

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The Gene Expression Profile of Peripheral Blood Mononuclear Cells from CA16-Infected Rhesus Neonates

Project description:The present work focuses on global gene expression of peripheral blood mononuclear cells in rhesus monkey infants with HFMD induced by CA16 infection at different time points. Genome-wide expression and analysis were performed by using Agilent whole genome microarrays and established bioinformatics tools. 931 signiM-oM-,M-^Acantly differentially expressed genes associated with 5 gene ontology categories, including cell communication, cell cycle, immune system process, regulation of transcription and metabolic process were identified. Subsequently, by mapping genes related to immune system process on KEGG pathways, the predominance of inflammation mediate by chemokine and cytokine signaling pathway and interleukin signaling pathway emerged. Ultimately, co-expression genes and their networks were analysised. Coxsackievirus A16 infection induced gene expression in rhesus monkey peripheral blood mononuclear cells was measured at 7-1st dpi, 3-2nd dpi, 7-2nd dpi, 14-2nd dpi. Three samples from two experiment donors and one control donor were performed at each time. To exclude the individual difference, mix the two experiment samples at each time, and mix the two control samples.

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Different microRNA Alterations Contribute to Diverse Outcomes Following EV71 and CA16 Infections: Insights from High-Throughput Sequencing in Peripheral Blood Mononuclear Cells of Rhesus Monkey

Project description:Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) are the predominant etiological agents of hand, foot, and mouth disease (HFMD) and both belong to the human enterovirus A species of the Picornaviridae family. These viruses share similar genetic homology, although the clinical manifestations of HFMD caused by the two viruses have some discrepancies. Furthermore, the underlying mechanisms leading to these differences remain unclear. microRNAs (miRNAs) participate in numerous biological or pathological processes, including host responses to viral infections, by targeting messenger RNAs (mRNAs) for translational repression or degradation. Here, we focused on differences in miRNA expression patterns in peripheral blood mononuclear cells (PBMCs) of rhesus monkeys infected with EV71 or CA16 at different time points using high-throughput sequencing technology. For the first time, this study demonstrated that EV71 and CA16 infection result in specific miRNA expression patterns in PBMCs.

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Transcriptome analysis of brainstem in gerbils with CA16-infection-induced HFMD

Project description:Transcriptome analysis of brainstem in gerbils with CA16-infection-induced HFMD

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Transcriptome analysis of brainstem in gerbils with EV71-infection-induced HFMD

Project description:Enterovirus 71 (EV71) is one of the major pathogens associated with human hand, foot, and mouth disease (HFMD) and in some countries this pathogen is the leading cause of this disease. We previously found that CA16 could induce neurological disorders in young gerbils, which could serve as a good animal model for studying the neuropathogenesis of CA16 infection. In this study, we found that 1039 genes were up-regulated and 299 genes were down-regulated in CA16-infected gerbils compared with control. Differentially expressed genes were clustered into functional pathways and the top five pathways according to the enrich factor were viral myocarditis, type 1 diabetes mellitus, toxoplasmosis, toll-like receptor signaling pathway and TNF signaling pathway. Our results provide novel insight into the neuropathogenesis of HFMD induced by CA16 infection.

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Project description:To compare MicroRNA expression in 16HBE infected with EV71 and CA16 we defined the following experimental groups: EV71-0h, EV71-6h, EV71-12h, CA16-0h, CA16-6h and CA16-12h

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Beijing Institute of Radiation Medicine Enterovirus Protein Array

Project description:Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are major causative agents for hand, foot and mouth disease (HFMD). In China, more than 70,756 children were infected and 40 died from the disease in recent years. This study aimed to develop a protein chip that can simultaneously detect and differentiate the antibodies induced by EV71 and CA16 simultaneously. The structure protein vp1 and vp3 from the two viruses were purified and spotted onto an aldehyde groupmodified glass slide at 1mg/ml. After that, the protein chip was reacted with the corresponding positive serum against these viruses, hybridized with a Cy3-labeled secondary antibody and scanned using the popular GenePix 4000B Microarray Scanner. In this study, Both IgG and IgM serum antibody to EV71 and CA16 were detected using protein Chip. The results showed that this method could hybridize specifically with the corresponding antibodies with strong signals and without cross-hybridization. The data also confirmed the proposed method's specificity, sensitivity, and convenience. In conclusion, this protein chip can be used to differentiate the antibodies induced by the EV71and CA16.

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Different microRNA Profiles Reveal the Diverse Outcomes Induced by EV71 and CA16 Infection in Human Umbilical Vein Endothelial Cells Using High-Throughput Sequencing

Project description:We performed comprehensive miRNA proﬁling in EV71- and CA16-infected human umbilical vein endothelial cells (HUVECs) at multiple time points using high-throughput sequencing. The results showed that 135 known miRNAs exhibited remarkable differences in expression. Of these, 30differentially expressed miRNAs presented opposite trends in EV71- and CA16-infected samples. Subsequently, we mainly focused on the 30 key differentially expressed miRNAs through further screening to predict targets.Gene ontology (GO) and pathway analysis of the predicted targets showed the enrichment of 14 biological processes, 9 molecular functions, 8 cellular components, and 85 pathways. The regulatory networks of these miRNAs with predicted targets, GOs, pathways, and coexpression genes were determined, suggesting that miRNAs display intricate regulatory mechanisms during the infection phase. Consequently, we specifically analyzed the hierarchical GO categories of the predicted targets involved in biological adhesion. The results indicated that the distinct changes induced by EV71 and CA16 infection may be partly linked to the function of the blood-brain barrier. Taken together, this is the first report describing miRNA expression profiles in HUVECs with EV71 and CA16 infections using high-throughput sequencing. Our data provide useful insights that may help to elucidate the different host-pathogen interactions following EV71 and CA16 infection and offer novel therapeutic targets for these infections.

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