Strand-Specific RNA Sequencing for wild type and Drpd3 yeast entering quiescence [RNA-seq]
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ABSTRACT: Strand-specific RNA sequencing was performed on wild type yeast in log phase, after diauxic shift, and after entry into quiescence with incorporation of external ERCC RNA spike-in controls to account for global changes in RNA abundance. We find that RNA profiles undergo at least two transitions: 1) From log-to-diauxic shift where stress response genes are induced and translational machinery is massively repressed. 2) From diauxic shift-to-quiescence, where global transcript abundance is repressed 15-fold. The transition from diauxic shift to quiescence was found to require Rpd3, as deletion of Rpd3 prevented the global repression of the transcriptome after the diauxic shift.
ORGANISM(S): Saccharomyces cerevisiae
PROVIDER: GSE67149 | GEO | 2015/08/20
SECONDARY ACCESSION(S): PRJNA279128
REPOSITORIES: GEO
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