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Generation of Integration-free Induced Neural Stem Cells from Mouse Fibroblasts

ABSTRACT: Recent studies demonstrated that fibroblasts could be converted into induced neural stem cells (iNSCs). However, the insertional mutation caused by random integration of viral vectors has been a major limitation of iNSCs for the future clinical translation. Here we show that non-viral transfection of episomal vectors encoding Brn4/Pou3f4, Sox2, Klf4, and c-Myc sufficiently generates iNSCs. The episomal vector mediated iNSCs closely resemble brain-derived NSCs as well as iNSCs generated by retrovirus in morphology, gene expression profile, epigenetic status, self-renewal capacity and both in vitro and in vivo differentiation capacity. The novel conversion protocol defined in the current study offers a method for generating integration-free iNSCs for the clinical research

ORGANISM(S): Mus musculus

PROVIDER: GSE67319 | GEO | 2015/03/27

SECONDARY ACCESSION(S): PRJNA279501

REPOSITORIES: GEO

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Generation of Integration-free Induced Neural Stem Cells from Mouse Fibroblasts

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