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DNA methylation profiling of human THP-1 monocytes stimulated with arachidonic acid (AA) or oleic acid (OA) and controls

ABSTRACT: High-density array-based DNA methylation profiling of human THP-1 monocytes stimulated for 24 h with 1, 10 or 100uM AA, or the same concentrations of OA, and unstimulated controls. The Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across approximately 485,000 CpGs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE67331 | GEO | 2015/03/27

SECONDARY ACCESSION(S): PRJNA279525

REPOSITORIES: GEO

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DNA methylation profiling of human THP-1 monocytes stimulated with arachidonic acid (AA) or oleic acid (OA) and controls

Project description:High-density array-based DNA methylation profiling of human THP-1 monocytes stimulated for 24 h with 1, 10 or 100uM AA, or the same concentrations of OA, and unstimulated controls. The Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across approximately 485,000 CpGs. Bisulphite converted DNA was hybridised to the Illumina Infinium 450k Human Methylation Beadchip

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Expression data from human THP-1 monocytes stimulated with increasing concentrations of arachidonic (AA) or oleic acid (OA).

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Arachidonic and oleic acid exert distinct effects on the DNA methylome.

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