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Interactions of C4 subtype metabolic activities and transport in maize are revealed through the characterization of dct2 mutants

ABSTRACT: We sequenced mRNA from from four segments along a developing 9 day old third maize leaf of WT W22 accession and ZmDCT2 KO (in W22 background - dct2-1::Ac) plants, to identify changes in gene expression in dct2-1::Ac as a response to the loss of ZmDCT2 function.

ORGANISM(S): Zea mays

PROVIDER: GSE67722 | GEO | 2015/08/30

SECONDARY ACCESSION(S): PRJNA280756

REPOSITORIES: GEO

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ZmDCT2 has a major role in the photosynthetic development of maize

Project description:We sequenced mRNA from from four segments along a developing 9 day old third maize leaf of WT W22 accession and ZmDCT2 KO (in W22 background - dct2-1::Ac) plants, to identify changes in gene expression in dct2-1::Ac as a response to the loss of ZmDCT2 function. The segments were referenced relative to the position of the ligule of leaf 2 as this point represents the source-sink boundary in a wild-type leaf and the transition from shaded leaf tissue to fully light exposed. Segment -4 was isolated at the base of the leaf blade that is photosynthetically inactive, -1, defined as the sink to source transition zone (just below the point of leaf emergence from the whorl), segment +4, from a fully light exposed and photosynthetically active region in the mid-leaf (maturation zone), and +10, the tip of the leaf and the terminally differentiated tissue

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Daily temperature cycles promote alternative splicing of RNAs encoding SR45a, a splicing regulator in maize

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