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Genome-wide maps of ParBF (SopB) binding along DNA in E. coli

ABSTRACT: We report the genome-wide analysis from chromatin immunoprecipitated DNA (ChIP-sequencing) at very high resolution (about 50 millions reads) of the DNA binding pattern of ParBF (SopB) of plasmid F

ORGANISM(S): Escherichia coli

PROVIDER: GSE67869 | GEO | 2015/09/04

SECONDARY ACCESSION(S): PRJNA281206

REPOSITORIES: GEO

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Genome-wide maps of ParBF (SopB) binding along DNA in E. coli

Project description:We report the genome-wide analysis from chromatin immunoprecipitated DNA (ChIP-sequencing) at very high resolution (about 50 millions reads) of the DNA binding pattern of ParBF (SopB) of plasmid F Differencial examination of ParBF binding by comparing input and IP (anti-SopB antibody) samples

2015-09-04 | E-GEOD-67869 | biostudies-arrayexpress

Genome-wide maps of ParBF (SopB) binding on plasmid F or E.coli chromosome carrying parSF

Project description:We report the genome-wide analysis from chromatin immunoprecipitated DNA (ChIP-sequencing) at very high resolution of the DNA binding pattern of ParBF (SopB) either on the full length plasmid F or on E. coli chromosome carrying the parSF centromere sequence. We also varied the intracellular ParBF concentration to discriminate between the several proposed mechanism of partition complexes assembly.

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ParBF DNA binding profiles over various supercoiling level and on linear DNA

Project description:We report the genome-wide analysis from chromatin immunoprecipitated DNA (ChIP-sequencing) of the DNA binding pattern of ParBF (SopB) of plasmid F. This study, performed in E. coli and Salmonella typhimurium, investigates the impact of DNA supercoiling on ParBF DNA binding profiles in vivo. We found that variation in DNA supercoiling does not significantly affect the ParB DNA binding profiles even on linear DNA.

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Project description:Genome-wide maps of ParBF (SopB) binding along DNA in E. coli

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Genome-wide maps of ParBF (SopB) binding on plasmid F or E.coli chromosome carrying parSF

Project description:Genome-wide maps of ParBF (SopB) binding on plasmid F or E.coli chromosome carrying parSF

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Project description:We report the MBD1 binding profiles in mouse dentate gyrus by sequencing MBD1 chromatin immunoprecipitated DNA

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