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Altered neurotrophin expression profiles in the nucleus of the solitary tract of spontaneously hypertensive rats

ABSTRACT: Our previous findings suggest that the nucleus of the solitary tract (NTS), a pivotal region for regulating the set-point of arterial pressure, exhibits abnormal inflammation in pre-hypertensive and spontaneously hypertensive rats (SHRs) together with elevated anti-apoptotic and low apoptotic factor levels compared with that of normotensive Wistar–Kyoto (WKY) rats. Whether this chronic condition affects neuronal growth and plasticity in the NTS remains unknown. To unveil the characteristics of the neurodevelopmental environment in the NTS of hypertensive rats, we investigated the gene expression profile of neurotrophins and their receptors in SHRs compared to that of normotensive rat WKY.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE67874 | GEO | 2015/04/15

SECONDARY ACCESSION(S): PRJNA281108

REPOSITORIES: GEO

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Altered neurotrophin expression profiles in the nucleus of the solitary tract of spontaneously hypertensive rats

Project description:Our previous findings suggest that the nucleus of the solitary tract (NTS), a pivotal region for regulating the set-point of arterial pressure, exhibits abnormal inflammation in pre-hypertensive and spontaneously hypertensive rats (SHRs) together with elevated anti-apoptotic and low apoptotic factor levels compared with that of normotensive Wistar–Kyoto (WKY) rats. Whether this chronic condition affects neuronal growth and plasticity in the NTS remains unknown. To unveil the characteristics of the neurodevelopmental environment in the NTS of hypertensive rats, we investigated the gene expression profile of neurotrophins and their receptors in SHRs compared to that of normotensive rat WKY. The NTS was dissected from the brain of 6 SHRs and 6 WKY rats and the total RNA was extracted. In both groups of rats (SHRs & WKY rats, n = 6 each), a total of 2 ug mRNA extracts from each NTS were pooled together, treated with RNase-free DNAse I (Invitrogen Life technologies) to remove any genomic contamination, and further purified using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions. Reverse transcription was subsequently performed on 1 ug total RNA using SuperArray’s RT2 First Strand Kit (SABiosciences); the resulting cDNA was submitted for real-time quantitative PCR reactions on RT2 ProfilerTM PCR array plates using Superarray RT2 SYBR Green qPCR Master Mix (SAbiosciences) and iCycler iQ thermal cycler (Bio-rad), following the manufacturer’s instructions. The experiment was performed in duplicate in each group.

2015-04-15 | E-GEOD-67874 | biostudies-arrayexpress

Gender differences in the cytokines and chemokines gene expression profiles within the medulla oblongata of SHRs

Project description:Medulla oblongata is a region of the brain containing multiple blood pressure regulation centers, including the nucleus of the solitary tract (NTS), a pivotal region for regulating the set-point of arterial pressure. Our previous findings suggest that the NTS of male pre-hypertensive rats and spontaneously hypertensive rats (SHRs) exhibits abnormal inflammatory condition compared with that of normotensive Wistar-Kyoto (WKY) rats. Females of SHRs exhibit a lower blood pressure than their male counterpart but the molecular mechanism is not well described. To unveil the gender differences in the central regulation of blood pressure, we investigated the gene expression profile of cytokines and chemokines in the medulla oblongata of female SHRs compared to that of their male counterparts.

2016-10-12 | GSE87746 | GEO

Gender differences of gene expression profiles in brain cardiovascular centers of WKY rats [nucleus of solitarii tract]

Project description:Arterial pressure (AP) is lower in pre-menopausal women than in men of similar age. Pre-menopausal women exhibit a lower sympathetic outflow and a greater baroreceptor reflex, however molecular mechanisms for the gender differences of AP regulation are still not well understood. Since the nucleus tractus solitarius (NTS), a pivotal region of the medulla oblongata for regulating the set-point of AP is strongly associated with the AP level, we hypothesized that a different neuronal functions at the level of the NTS between men and women could contribute to the gender difference in cardiovascular homeostasis. Females Spontaneous Hypertensive Rats (SHRs) clearly exhibit lower AP levels than their male counterparts at similar age and their NTS is characterized by a specific gene expression profile. This gender dependence of AP level is less marked in the normotensive strain, Wistar Kyoto rats (WKY). In this study, we investigated the gender-dependent gene expression profile of the NTS of WKY by using microarray technique.

2018-01-30 | GSE107658 | GEO

Excessive leukotriene B4 in nucleus tractus solitarii is pro-hypertensive in spontaneously hypertensive rats.

Project description:We have used Affymetrix microarray-driven gene profiling to comprehensively describe the expression of mRNAs in the nucleus tractus solitarii (NTS) in the adult male spontaneously hypertensive rat (SHR) as compared to its normotensive parental Wistar-Kyoto (WKY) strain. Keywords: Gene expression NTS was dissected from hypertensive (SHR) and normotensive (WKY) strains of rat. For each structure, 10 independent Affymetrix Genechip 230 2.0 experiments were performed (5 SHR, 5 WKY), with each chip representing an independent biological replication (n=5).

2013-03-01 | E-GEOD-8796 | biostudies-arrayexpress

The effect of trimethylamine N-oxide on the metabolism of visceral white adipose tissue in spontaneously hypertensive rat

Project description:Male SHRs and normotensive Wistar-Kyoto(WKY) rats were purchased from Vital River (Vital River Laboratory Animal Technology Co., Ltd, Beijing, China). Briefly, eightteen SHRs aged seven to eight weeks old were randomly assigned to one of three groups: water group (drinking tap water), water containing 333 mg/L or 1g/L TMAO group (TMAO, product number: T1362, Tokyo Chemical Industry, Japan). Six WKY rats of the same age were used as normotensive controls. In this study, WKY rats (n=6), SHR (n=6), and SHR treated with 1g/L TMAO (n=6) were uesd for experiments.

2022-08-17 | GSE188336 | GEO

Gender differences of gene expression profiles in brain cardiovascular centers of WKY rats [hypothalamus]

Project description:Arterial pressure (AP) is lower in pre-menopausal women than in men of similar age. Pre-menopausal women exhibit a lower sympathetic outflow and a greater baroreceptor reflex, however molecular mechanisms for the gender differences of AP regulation are still not well understood. Since the hypothalmus is strongly functionnaly connected to the nucleus tractus solitarius (NTS), a pivotal region of the medulla oblongata for regulating the set-point of AP, we hypothesized that a different neuronal functions at the level of hypothalamus between men and women could contribute to the gender difference in cardiovascular homeostasis. Females Spontaneous Hypertensive Rats (SHRs) clearly exhibit lower AP levels than their male counterparts at similar age and their hypothalamus is characterized by a specific gene expression profile. This gender dependence of AP level is less marked in the normotensive strain, Wistar Kyoto rats (WKY). In this study, we investigated the gender-dependent gene expression profile of the hypothalamus of WKY rats by using microarray technique.

2018-01-30 | GSE107659 | GEO

miRNA Profiling of Exosomes from Spontaneous Hypertensive Rats by Next-Generation Sequencing

Project description:The role and miRNA expression profile of exosomes in hypertension remain largely unknown, therefore, next generation sequencing was used to define the miRNA expression profile of plasma exosomes in spontaneously hypertensive rats (SHRs), the most widely used animal model of human essential hypertension, and their controls, normotensive Wistar-Kyoto rats (WKYs). Results revealed that percentages of miRNA in the total small RNA isolated from WKYs and SHRs were not significantly different. Twenty-seven miRNAs were significantly differentially expressed (DE) between WKY and SHR exosomes, among which 23 were upregulated and 4 were downregulated in SHR exosomes compared with WKY exosomes. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis of top 10 DE miRNAs identified hypertension-specific target genes/signaling pathways. In conclusion, our findings indicate the selective packing of miRNA cargoes into exosomes under hypertensive status, while facilitating the development of potential targets for the diagnosis, prevention and treatment of hypertension.

2020-10-30 | GSE106333 | GEO

Genome-wide Profiling of Long Noncoding RNA Expression Patterns in Thoracic Aorta from Spontaneously Hypertensive Rats.

Project description:We used lncRNA-mRNA micro-array to analyze the transcriptome in the thoracic aorta tissue of 24 wees-old WKY and spontaneously hypertensive rats (SHRs).

2021-01-01 | GSE145854 | GEO

Excessive leukotriene B4 in nucleus tractus solitarii is pro-hypertensive in spontaneously hypertensive rats.

2013-03-01 | GSE8796 | GEO

Transcription profiling of rat kidney from spontaneously hypertensive rats vs. controls

Project description:Development of renal transcriptome in spontaneously hypertensive rats (SHR) as compared to normotensive wistar kyoto rats (WKY) from birth to old age.

2008-04-30 | E-MEXP-1550 | biostudies-arrayexpress

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