Genomics

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Genome-wide identification of CCA1-bound genes in Arabidopsis by ChIP-Seq


ABSTRACT: LHY and CCA1 encode single MYB transcription factors, involved in circadian clock. However, direct target genes of LHY and CCA1 in a genomic scale were largely unknown. To reveal bound genes by CCA1, chimeric protein CCA1-FLAG was expressed under CCA1 promoter in cca1 lhy (CCA1pro:CCA1-FLAG/ cca1 lhy). ChIP was performed using anti-FLAG antibody (F3165; SIGMA), which was bound to Dynabeads Protein G (100-03D; Life Technologies), and ChIP DNA were analyzed by IonPGM or Illumina GAII.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE67903 | GEO | 2016/03/11

SECONDARY ACCESSION(S): PRJNA281176

REPOSITORIES: GEO

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