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Long RNAs expression profiling of 20 Italian Large White pig backfat

ABSTRACT: We report the application of Illumina RNA sequencing for characterization and discovery of genes and transcripts in Italian Large Whtie pig backfat tissue.

ORGANISM(S): Sus scrofa

PROVIDER: GSE68007 | GEO | 2016/03/15

SECONDARY ACCESSION(S): PRJNA281483

REPOSITORIES: GEO

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Long RNAs expression profiling of 20 Italian Large White pig backfat

Project description:We report the application of Illumina RNA sequencing for characterization and discovery of genes and transcripts in Italian Large Whtie pig backfat tissue. RNAs sequencing for long RNA quantification, discovery, characterisation and differential expression evaluation.

2016-03-15 | E-GEOD-68007 | biostudies-arrayexpress

Short RNAs expression profiling of two Italian Large White pig backfat

Project description:We report the application of Illumina short RNA sequencing for characterization and discovery of miRNAs and moRNAs in two Italian Large White pig backfat tissue.

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Identification of differentially expressed small RNAs and prediction of target genes in Italian Large White pigs with divergent backfat deposition

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Sus scrofa breed:Italian Large White

Project description:Long RNAs expression profiling of 20 Italian Large White pig backfat

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Comparison between Backfat Tissue of Fat Type Pig and Lean Type Pig, among developmental phases

Project description:The objective of this study was to identify key genes associated with porcine muscle growth and adipose metabolism which different expression in porcine backfat tissue between Fat Type Pig(Taihu pig) and Lean Type Pig (Landrace), among developmental phases(Month 1,2,3,4,5). The gene expression analyses will increase understanding of impact factors of pork quality by identifying key genes and pathways controlling backfat development. Relative real-time RT-PCR was used to confirm differential expression of 3 different expression genes(ME,SCD and UCP3) which normalized by 3 housekeep genes(ACTB, TBP and TOP2B). Keywords: time course and breed comparison

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Comparative methylation and RNAseq expression analysis on CpG context to identify genes involved in backfat vs liver diversification in Nanchukmacdon pig

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Identification of differentially expressed small RNAs and prediction of target genes in Italian Large White pigs with divergent backfat deposition

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Project description:Purpose: Analysis of the effect of different fats and amonut of cDDGS in the feedstuff on miRNA expression in porcine backfat Methods: miRNA-seq analysis was performed on backfat samples collected from 24 male and female crossbred fatteners originating from sows (Polish Landrace × White Large Polish) mated with a boar (Duroc × Pietrain) divided into four dietary groups: 7-cDDGS+rapeseed oil (group I), n=6 (+cDDGS+rapeseed oil -group II), n= 6 (+cDDGS+beeftallow -group III),n=5 (+cDDGS+coconut oil -group IV). The miRNA libraries were constructed from total RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer protocol. The quantification of the obtained libraries was performed on a Qubit 2.0 spectrophotometer (Invitrogen, Life Technologies), while a quality control on a TapeStation 2200 instrument (D1000 ScreenTape; Agilent). 100 single-end cycle sequencing was performed on the HiScanSQ platform (Illumina) with the use of TruSeq SR Cluster Kit v3- CBOT-HS and TruSeq SBS Kit v 3 - HS (Illumina). MicroRNA differentially expressed between dietary groups were identified with the DESeq2 software. Results: The comparison of miRNA profiles between dietary groups showed The highest number of miRNAs with altered expression was identified in the comparison of animals fed the diet containing cDDGS and coconut oil (group IV) with animals from the –cDDGS + rapeseed oil (group I) (37 miRNA, p adjusted <0.01). Moreover, in comparison between the group IV and groups III and II , 29 (12 upregulated and 17 downregulated in +cDDGS+coconut oil group) and 28 (10 upregulated and 18 downregulated in +cDDGS+coconut oil group) miRNAs were identified, respectively (p adjusted <0.1) Conclusions: Obtained results suggest that coconut oil induces changes in miRNA profile of backfat in pigs.

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Pig Longissimus muscle: comparison between Basque and Large White breeds

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