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HeLa cells: Control vs. GPRC5A depletion at different stages of adhesion to Matrigel

ABSTRACT: Transcriptional profiling of HeLa cervical carcinoma cells comparing cells with siRNA- or shRNA-mediated depletion of GPRC5A vs. cells carrying non-targeting siRNA or shRNA after 45 min and 6 h of adhesion to 10% Matrigel matrix. The goal was to determine the effect of GPRC5A depeltion on the global gene expression following the adhesion process.

ORGANISM(S): Homo sapiens

PROVIDER: GSE68338 | GEO | 2016/12/31

SECONDARY ACCESSION(S): PRJNA282470

REPOSITORIES: GEO

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GPRC5A regulates keratinocyte adhesion and migration through nuclear translocation of its C-terminus region

Project description:G-Protein Coupled Receptor, Class C, Group 5, Member A (GPRC5A) is well-documented in lung and various epithelial cancers. However, its role in the skin remains unexplored. In this study, we investigated the function of this receptor in skin biology and our research demonstrated that its expression responds to mechanical substrate changes in human primary keratinocytes. Furthermore, we observed GPRC5A reinduction during wound healing at the leading edges in an ex vivo burn model, coinciding with the translocation of its C-terminal region into the nucleus. We identified the cleavage site of GPRC5A by N-TAILS analysis, and cathepsin G was characterized as responsible for proteolysis in cultured cells. To gain a deeper understanding of GPRC5A's role in keratinocyte, we performed GPRC5A knockdown in N/TERT-1 cells using short-hairpin RNA. Our findings strongly suggest a close association between GPRC5A and adhesion regulation pathways, but also demonstrate that GPRC5AKD enhanced cell adhesion while reducing cell migration and differentiation. Importantly, these effects were reversed by adding a recombinant polypeptide mimicking the C-terminal region of GPRC5A. Overall, our study reveals an unexpected role of GPRC5A in regulating keratinocyte behavior, implicating its C-terminal region translocation into the nucleus. These results open up interesting strategic pathways for wound healing.

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GPRC5A regulates keratinocyte adhesion and migration through nuclear translocation of its C-terminus region

Project description:GPRC5A regulates keratinocyte adhesion and migration through nuclear translocation of its C-terminus region

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Expression data from Gprc5a wild type or knockout mouse tracheal epithelial cells (MTECs)

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Homo sapiens

Project description:HeLa cells: Control vs. GPRC5A depletion at different stages of adhesion to Matrigel

| PRJNA282470 | ENA

Elevation of GPRC5A expression in colorectal cancer promotes tumor progression through VNN-1 induced oxidative stress

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