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Structural evidence for Scc4-dependent localization of cohesin loading

ABSTRACT: Purpose: The goal of this study is to look at differences in genome wide localisation of Scc1 cohesin between wild type and scc4-m35 in metaphase arrested cells. Method: Wild-type (AM1145), wild type constructed in the same way as the mutant strain (carrying a HIS3 marker) (AM15540) and scc4-m35 mutant (AM15537) cells carrying SCC1-6HA were synchronised with a-factor and arrested in mitosis by treatment with nocodazole/benomyl for 2 hr. Samples were harvested, anti-HA ChIP was performed and input and IP samples were sequenced for the three strains. Results: All 16 centromeres were specifically depleted of Scc1 in the scc4-m35 background. Scc1 depletion extended roughly 10 kilobases to either side of the core centromere but not to chromosome arms

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE68573 | GEO | 2015/06/05

SECONDARY ACCESSION(S): PRJNA283124

REPOSITORIES: GEO

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Structural evidence for Scc4-dependent localization of cohesin loading

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