Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

An Nfic-Hedgehog Signaling Cascade Regulates Tooth Root Development

ABSTRACT: This study aimed to perform transcriptome profiling of Nfic-/- and corresponding control tooth germ at root initiation stage to identify differentially expressed for key regulators of root development. Coordination between the Hertwig’s Epithelial Root Sheath (HERS) and apical papilla (AP) is crucial for proper root development process. The Hedgehog (Hh) signaling pathway and Nfic are both involved in tooth root development.

ORGANISM(S): Mus musculus

PROVIDER: GSE69054 | GEO | 2017/08/29

SECONDARY ACCESSION(S): PRJNA284406

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

An Nfic-hedgehog signaling cascade regulates tooth root development.

Project description:Coordination between the Hertwig's epithelial root sheath (HERS) and apical papilla (AP) is crucial for proper tooth root development. The hedgehog (Hh) signaling pathway and Nfic are both involved in tooth root development; however, their relationship has yet to be elucidated. Here, we establish a timecourse of mouse molar root development by histological staining of sections, and we demonstrate that Hh signaling is active before and during root development in the AP and HERS using Gli1 reporter mice. The proper pattern of Hh signaling activity in the AP is crucial for the proliferation of dental mesenchymal cells, because either inhibition with Hh inhibitors or constitutive activation of Hh signaling activity in transgenic mice leads to decreased proliferation in the AP and shorter roots. Moreover, Hh activity is elevated in Nfic(-/-) mice, a root defect model, whereas RNA sequencing and in situ hybridization show that the Hh attenuator Hhip is downregulated. ChIP and RNAscope analyses suggest that Nfic binds to the promoter region of Hhip. Treatment of Nfic(-/-) mice with Hh inhibitor partially restores cell proliferation, AP growth and root development. Taken together, our results demonstrate that an Nfic-Hhip-Hh signaling pathway is crucial for apical papilla growth and proper root formation. This discovery provides insight into the molecular mechanisms regulating tooth root development.

| S-EPMC4631759 | biostudies-literature

SCAPs Regulate DFSC Differentiation via the Osteoglycin-Hedgehog Pathway

Project description:The developing root apical complex, including the Hertwig’s epithelial root sheath, apical papilla, and dental follicle (DF), is the germinal center of root development wherein the DF constantly develops into periodontal tissues. However, whether the DF development is regulated by the adjacent apical papilla is largely unknown. In this study, using a transwell system in vitro, we found that stem cells from the apical papilla (SCAPs) inhibited the differentiation but maintained the stemness of dental follicle stem cells (DFSCs). Furthermore, high-throughput transcriptome sequencing revealed that the Hedgehog (Hh) pathways were significantly downregulated in DFSCs when co-cultured with SCAPs. Upregulation or downregulation of the Hh pathway could activate or inhibit the multidirectional differentiation of DFSCs, respectively. By secreting osteoglycin (OGN), the SCAPs regulated the stemness and multidirectional differentiation of DFSCs via the OGN-Hh pathway.

2022-10-25 | GSE209650 | GEO

The cellular basis for epithelial-mesenchymal interaction during mouse tooth development

Project description:In this study, using mouse molar as the model, we developed a dual fluorescence reporter mouse to precisely track and analyze dental epithelium and mesenchyme at single-cell resolution from early embryonic to postnatal stages. Moreover, we constructed the virtual molar explorer (VMEx) to spatially map 15,967 molar-expressed genes and identified that Msx1+ Sdc1+ marked the developing dental papilla while surrounded by Msx1+ Sdc1- molar niche. Through tooth germ reconstitution and organoid culture in vitro and kidney capsule transplantation in vivo, we provided evidence that the Msx1+ Sdc1- dental follicle cells might function as the tooth organizers that promoted epithelium survival and tooth germ organization. Furthermore, the appearance of Msx1+ Sdc1+ dental papilla cells relied on the interaction between dental epithelium and Msx1+ Sdc1- dental follicle cells. Together, our results revealed the cellular dynamics of tooth development in mice and identified that the dental follicle might be the key driver of epithelial-mesenchymal interaction and tooth morphogenesis.

2022-11-01 | GSE162413 | GEO

RNA-Seq of wild type and Nfic-/- mice pancreata

Project description:To assess the role of Nfic in pancreatic development and homeostasis we used constitutive Nfic knockout mice.

2023-05-24 | GSE126907 | GEO

Mus musculus

Project description:An Nfic-Hedgehog Signaling Cascade Regulates Tooth Root Development

| PRJNA284406 | ENA

NFIC overexpression perturbs normal haematopoeisis by promoting myeloid differentiaon

Project description:Nuclear factor I-C belongs to a family of CTF binding transcription factor which is known for its role cellular differentiation. In our recent study we identified increased expression of NFIC in. AML samples as compared to normal haematpoitic stem and progenitor cells (HSPCs). We also found that overexpression of NFIC in cord blood derived HSPCs perturbed normal haematopoiesis by increasing monocyte production. Therefore in this analysis we aimed to identify the effect of NFIC overexpression in normal HSPCs at single cell level using single cell RNA (scRNA) sequencing. Cluster analysis based on the cellular and functional identities, identified eight clusters according to their terminal differentiation as monocytes, erythrocytes, dendritic cells (DC), megakaryocytes, neutrophils, megakaryocyte-erythroid progenitor cells, basophil/mast progenitor cells and eosinophils. We found that NFIC overexpressing CD34+ HSPCs had increased percentage of monocytes as compared to control. To understand the molecular basis of this, we focussed our analysis on monocytes to determine changes in mRNA expression following NFIC overexpression. We identified 164 and 88 significantly up-regulated and down-regulated genes, respectively (p<0.05, FDR<0.05) in NFIC overexpressing monocytes as compared to control. Pathway analysis identified enrichment in genes involved in mTOR signalling, cancer cell death and survival, energy metabolism pathways and oxidative phosphorylation. Therefore our scRNA suggested that NFIC play a role in monocytic growth development and its overexpression may lead to monocytic bias in normal haematopoiesis.

2023-01-04 | GSE200456 | GEO

Notum Regulates the Cusp and Root Patterns in Mouse Molar

Project description:Notumis a direct target of Wnt/β-catenin signaling and plays a crucial role as a Wnt inhibitor within a negative feedback loop. In the tooth, Notum is known to be expressed in odontoblasts, and severe dentin defects and irregular tooth roots have been reported in Notum-deficient mice. However, the precise expression pattern of Notum in early tooth development, and the role of Notum in crown and root patterns remain elusive. In the present study, we identified a novel Notum expression in primary enamel knot (EK), secondary EKs, and dental papilla during tooth development. Notum-deficient mice exhibited enlarged secondary EKs, resulting in broader cusp tips, altered cusp patterns, and reduced concavity in crown outline. These alterations in crown outline led to a reduction in cervical tongue length, thereby inducing root fusion in Notum-deficient mice. Overall, these results suggest that the size of secondary EK size, regulated by the Wnt/Notum negative feedback loop, has a significant impact on the patterns of crown and root during tooth morphogenesis.

2024-06-10 | GSE255946 | GEO

Expression data of microRNA from human stem cells from apical papilla

Project description:Osteo/odontogenic differentiation is a key process of human stem cells from apical papilla (SCAP) in tooth root development. Emerging evidence indicates microRNAs (miRNAs) play diverse roles in osteogenesis. However, their functions in osteo/odontogenic differentiation of SCAP remain to be elucidated. We used miRNA microarray to analysis differentially expressed miRNAs in order to investigate the role of miRNAs in SCAP osteo/odontogenic differentiation and underlying mechanism.

2021-01-01 | GSE154466 | GEO

microRNA expression during tooth development

Project description:microRNA expression during tooth development

| PRJNA594040 | ENA

Keystone genes for tooth development

Project description:Keystone genes for tooth development

| PRJNA596203 | ENA