Phloroglucinol functions as an intercellular chemical messenger with broad transcriptional effects in Pseudomonas protegens
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ABSTRACT:
ORGANISM(S): Pseudomonas protegens
PROVIDER: GSE69178 | GEO | 2015/10/01
SECONDARY ACCESSION(S): PRJNA284756
REPOSITORIES: GEO
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Project description:Biological control is a promising approach to control diseases caused by Pythium species. Unusually for a single genus, the Pythium genus also includes species that can antagonise Pythium plant pathogens, such as Pythium oligandrum. These Pythium plant pathogens are commonly found in the soil such as the broad host-range pathogen Pythium myriotylum and cause various diseases of important crops. While P. oligandrum genes expressed in the interaction with oomycete plant pathogens have been identified previously, the transcriptional response of an oomycete plant pathogen to P. oligandrum has not been investigated. An isolate of P. oligandrum, GAQ1, recovered from soil could antagonise P. myriotylum in a plate-based confrontation assay. The P. oligandrum isolate had a strong disease control effect on soft-rot of ginger caused by P. myriotylum. We investigated the transcriptional interaction between P. myriotylum and P. oligandrum. As part of the transcriptional response of P. myriotylum to the presence of P. oligandrum, putative effector genes such as a sub-set of Kazal-type protease inhibitors were strongly upregulated. P. myriotylum cellulases and elicitin-like putative effectors were also upregulated. In P. oligandrum, cellulases, peroxidases, proteases and NLP effectors were upregulated. The transcriptional response of P. myriotylum suggests clear features of a counter-attacking strategy that may contribute to the variable success and durability of biological attempts to control diseases caused by Pythium species. Whether aspects of this counter-attack could inhibit aspects of this virulence of P. myriotylum is another interesting aspect for future studies.
2021-10-28 | GSE179387 | GEO
Project description:Contrary to the relative wealth of information regarding pathogen defense responses in aboveground plant parts, little is known about the mechanistic basis and regulation of plant immunity in root tissues. Aiming to further our fundamental understanding of root immune responses, we have investigated the interaction between rice and one of its major root pathogens, the oomycete Pythium graminicola. The specificic objectives of this study were twofold: i) to disentangle the molecular and genetic basis of the rice-Pythium interaction by comparing the transcriptome of rice roots at different times after inoculation with a highly virulent Pythium strains, and ii) to offer fundamental insights into the genetic architecture and regulation of rice disease resistance pathways operative in root tissue and to identify the molecular players controlling the possible nodes of convergence between these resistance conduits
2011-10-04 | GSE32582 | GEO
Project description:Contrary to the relative wealth of information regarding pathogen defense responses in aboveground plant parts, little is known about the mechanistic basis and regulation of plant immunity in root tissues. Aiming to further our fundamental understanding of root immune responses, we have investigated the interaction between rice and one of its major root pathogens, the oomycete Pythium graminicola. The specificic objectives of this study were twofold: i) to disentangle the molecular and genetic basis of the rice-Pythium interaction by comparing the transcriptome of rice roots at different times after inoculation with a highly virulent Pythium strains, and ii) to offer fundamental insights into the genetic architecture and regulation of rice disease resistance pathways operative in root tissue and to identify the molecular players controlling the possible nodes of convergence between these resistance conduits Comparison between P. graminicola- and mock-infected rice roots. Two treatments (infected and non-infected) x three timepoints (1, 2 and 4 days post inoculation) x three biological replicates
2011-10-03 | E-GEOD-32582 | biostudies-arrayexpress
Project description:Pythiosis is a deadly infectious disease of humans and animals living in tropical and subtropical countries. The causative agent is the oomycete Pythium insidiosum. Treatment of pythiosis is challenging. Therefore, protein profiling could generate the important information for further drug development.
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Project description:Next to their essential roles in plant growth and development, phytohormones play a central role in plant immunity against pathogens. In this study we examined the role of hormones in the antagonism of the plant-pathogenic oomycete Pythium arrhenomanes against the root-knot nematode Meloidogyne graminicola in rice roots. Hormone measurements and gene expression analyses showed that the jasmonate (JA) pathway is induced early upon P. arrhenomanes infection. Exogenous application of methyl-jasmonate (MeJA) on the plant confirmed that JA is needed for basal defence against both P. arrhenomanes and M. graminicola in rice. Whereas M. graminicola suppresses root JA levels to increase host susceptibility, Pythium inoculation boosts JA accumulation up to levels that can no longer be repressed by the nematode in double-inoculated plants. Exogenous MeJA supply phenocopied the defence-inducing capacity of P. arrhenomanes against the root-knot nematode, whereas the antagonism was weakened in JA-insensitive mutants. Transcriptome analysis confirmed upregulation of JA biosynthesis and signalling genes upon P. arrhenomanes infection, and additionally revealed induction of genes involved in biosynthesis of diterpenoid phytoalexins, consistent with strong activation of the gene encoding the JA-inducible transcriptional regulator DITERPENOID PHYTOALEXIN FACTOR. Next to that, our results provide evidence for induced expression of genes encoding ERF83, and related PR proteins, as well as auxin depletion in P. arrhenomanes infected rice roots, which potentially further contributes to the reduced nematode susceptibility seen in double-infected plants.
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Project description:P. yezoensis is an economically important marine crop and highly used seafood in China containing a high number of proteiP. yezoensis is an economically important marine crop and highly used seafood in China containing a high number of proteins. An oomycete, known as Pythium porphyrae, causes the red rot disease that seriously damages Pyropia farms every year in China, Korea, and Japan. To investigate the pathogen responsive proteins after the artificial infection of Pyropia with (P. porphyrae) oomycetes spores, an iTRAQ-based proteomic analysis was performed. A total of 762 differentially expressed proteins (DEP’s) were identified from which 378 proteins were highly expressed and 284 proteins were found to be low expressed. A large number of differentially expressed proteins were identified, which are involved in disease stress, carbohydrate metabolism, photosynthetic activity, and amino acid pathways as annotated in the Kyoto Encyclopedia of Genes and Genomes KEGG database. Our results showed that Pyropia resisted infection by inhibiting photosynthesis, energy and carbohydrate metabolism pathways, as supported by the change in the expression level of related proteins. Thus, the current research data provide an overall summary of the red algae response to pathogen infection. The present study could assist in a better understanding of the mechanisms behind infection resistance in P. yezoensis as well as improve the breeding of Pythium infection tolerant macroalgaens. An oomycete, known as Pythium porphyrae, causes the red rot disease that seriously damages Pyropia farms every year in China, Korea, and Japan. To investigate the pathogen responsive proteins after the artificial infection of Pyropia with (P. porphyrae) oomycetes spores, an iTRAQ-based proteomic analysis was performed. A total of 762 differentially expressed proteins (DEP’s) were identified from which 378 proteins were highly expressed and 284 proteins were found to be low expressed. A large number of differentially expressed proteins were identified, which are involved in disease stress, carbohydrate metabolism, photosynthetic activity, and amino acid pathways as annotated in the Kyoto Encyclopedia of Genes and Genomes KEGG database. Our results showed that Pyropia resisted infection by inhibiting photosynthesis, energy and carbohydrate metabolism pathways, as supported by the change in the expression level of related proteins. Thus, the current research data provide an overall summary of the red algae response to pathogen infection. The present study could assist in a better understanding of the mechanisms behind infection resistance in P. yezoensis as well as improve the breeding of Pythium infection tolerant macroalgae
2018-12-03 | PXD009363 | Pride
Project description:We report that phosphatidylglycerol (PG) biosynthesis in plastid is required for plastid gene expression mediated by plastid-encoded RNA polymerase and light-induced expression of nuclear-encoded photosynthesis-associated genes. A transcription factor GOLDEN-LIKE1 was also found to be involved in the downregulation of nuclear photosynthesis genes in responce to PG deficiency.
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Project description:The goal of this study was to determine how androgen receptor inhibition alters transcriptional programs in prostate cancer cells. LNCaP prostate cancer cells were grown in 10% charcoal-stripped serum (CSS) supplemented with 0.5 nanomolar dihydrotestosterone (DHT), in CSS without DHT modeling castration, with CSS + DHT but in the presence of 10 micromolar enzalutamide, or in CSS without DHT (castrated) and in the presence of enzalutamide for 72 hours. Analysis shows that androgen receptor target genes are reduced with castration, enzalutamide or the combination of castration + enzalutamide.
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