Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

B-cell response following malaria infection in WT and miR-155 KO mice

ABSTRACT: Analysis of the transcriptome pattern of splenic B lymphocytes after malaria infection in WT and miR-155 KO mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE69188 | GEO | 2022/01/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Spleen response following malaria infection in WT and miR-155 KO mice.

Project description:Analysis of the transcriptome pattern of spleen after maralia infection in WT and miR-155 KO mice.

2022-01-01 | GSE69931 | GEO

RNA-seq analysis of withdrawal of miR-155 hyperexpression in miR-155-addicted tumors

Project description:Overexpression of miR-155 in hematological tissue leads to the onset of lymphoma, and Tet-off shutdown of this overexpression reverses the disease phenotype in mir-155LSLtTA mice. This study compares the gene expression profiles of tumors with miR-155 overexpression and withdrawal. Examination of mRNA from 2 conditions: miR-155 overexpressing tumors and miR-155 overexpressing tumors from mice exposed to doxycycline (DOX) for 16hrs prior to harvest; samples in triplicate; tumors generated in flanks of nude mice by subcutaneous injection of splenic cells from diseased mir-155LSLtTA mice.

2015-01-30 | E-GEOD-61851 | biostudies-arrayexpress

miR-155 KO in human dendritic cells

Project description:In response to inflammatory stimulation, dendritic cells (DCs) have a remarkable pattern of differentiation (maturation) that exhibits specific mechanisms to control immunity. Here, we show that in response to Lipopolysaccharides (LPS), several microRNAs (miRNAs) are regulated in human monocyte-derived dendritic cells. Among these miRNAs, miR-155 is highly up-regulated during maturation. Using LNA silencing combined to microarray technology, we have identified the Toll-like receptor / interleukin-1 (TLR/IL-1) inflammatory pathway as a general target of miR-155. We further demonstrate that miR-155 directly controls the level of important signal transduction molecules. Our observations suggest, therefore, that in mature human DCs, miR-155 is part of a negative feedback loop, which down-modulates inflammatory cytokine production in response to microbial stimuli. We devised a strategy to functionally inhibit the mature form of miR-155 with the aim of identifying the signaling pathways controlled by miR-155 during DC maturation. A LNA-modified oligonucleotide, specifically designed for miR-155 knockdown (anti-miR-155 LNA) was introduced in moDCs by nucleofection prior LPS stimulation. 24h after transfection, a reduction in miR-155 levels of 8- and 32-fold was observed by qPCR in immature and mature moDCs respectively. A comparative microarray analysis (Affymetrix U133 2.0 chip) was performed among miR-155 silenced (anti-miR-155 LNA) and control transfected (scramble LNA) moDCs, exposed or not to LPS. Thus, four samples were totally analyzed. As expected from the limited expression of miR-155 in non-activated DCs, little variation in mRNA expression (177 probe sets differentially expressed employing a cut-off of 1.5) was detected upon miR-155 silencing. Conversely, in LPS-activated cells many mRNAs (1324 probe sets, 770 up-regulated and 554 down-regulated) were affected by miR-155 inhibition.

2009-03-04 | E-GEOD-13296 | biostudies-arrayexpress

Expression data from mouse B Cells with mir-155 KO and WT

Project description:We used microarrays to investigate the global changes of gene expression in B cells of mir-155 Knockout mice. Analysis of mir-155 knockout cultured and activated B Cells versus wild-type cultured and activated B Cells, by microarray profiling

2013-12-03 | E-GEOD-52940 | biostudies-arrayexpress

Expression data from mouse B Cells with mir-155 KO and WT

Project description:We used microarrays to investigate the global changes of gene expression in B cells of mir-155 Knockout mice.

2013-12-03 | GSE52940 | GEO

miR-155 KO in human dendritic cells

2009-03-04 | GSE13296 | GEO

GM-CSF cultured and LPS stimulated WT and miR-155 KO DC

Project description:Analyze the transcriptome pattern of miR-155 deficient BMDC in both steady-state and inflammatory conditions, in comparison to WT counterparts.

2022-01-01 | GSE40027 | GEO

Kinetics of M-CSF cultured and LPS stimulated WT and miR-155 KO Macrophages.

Project description:Analysis of the transcriptome pattern of miR-155 deficient Bone marrow-derived macrophages (BMMΦ) in both steady-state and inflammatory conditions after 6-, 12- and 24 h, in comparison to their WT counterparts.

2022-01-01 | GSE40029 | GEO

Gene profile of Wt and miR-155-/- Tfh cells

Project description:Differential gene expression profile of Tfh and non-Tfh cells from both Wt and miR-155-/- mice spleens.

2014-12-09 | GSE58371 | GEO

Gene profile of Wt and miR-155-/- Tfh cells

Project description:Differential gene expression profile of Tfh and non-Tfh cells from both Wt and miR-155-/- mice spleens. Wt and miR-155-/- mice were immunized with OVA. 8 days post immunization, CD4+CXCR+PD1+ Tfh cells and CD4+CXCR5-PD1- non Tfh cells were sorted from mice spleens for analyses.

2014-12-09 | E-GEOD-58371 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data