Gene expression profile of A549 cells cultured with novel 3D cell culture method
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ABSTRACT: Preclinical cancer drug discovery efforts have employed two-dimensional (2D)-cell-based assay models, which fail to forecast in vivo efficacy and contribute to a lower success rates of clinical approval. Three-dimensional (3D) cell culture models are recently expected to bridge the gap between 2D and in vivo models. We have developed novel 3D culture method that improves the growth of spheroid-forming cancer cells under anchorage-independent condition by leveraging a feature of FP001. Gene microarrays were used to observe the global gene expression in A549 cells cultured with normal adhesion plate (2D, control) or with low adhesion plate (+FP001) and identified distinct classes of up or down-regulated genes.
ORGANISM(S): Homo sapiens
PROVIDER: GSE69477 | GEO | 2015/07/31
SECONDARY ACCESSION(S): PRJNA285682
REPOSITORIES: GEO
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