Genomics

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Comparison of endogenous tRNA expression levels from cells expressing and sgRNA from either a U6 or tRNA promoter


ABSTRACT: The goal of this study was to compare the endogenous tRNA expression levels from cells transduced with a lentiviral vector encopding Cas9 and an sgRNA from a U6 or a Glutamine tRNA promoter. Using high-throughput sequencing methods (Illumina Hi-Seq 2000) we show that endogenous tRNA expression levels are not perturbed by expression of an sgRNA from a human tRNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE69720 | GEO | 2015/09/09

SECONDARY ACCESSION(S): PRJNA286739

REPOSITORIES: GEO

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